| Project/Area Number |
26430144
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Tumor diagnostics
|
|---|
| Research Institution | Saga University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
佐藤 明美 佐賀大学, 医学部, 助教 (20568357)
中村 朝美 佐賀大学, 医学部, 助教 (90457490)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KIMURA SHINYA 佐賀大学, 医学部, 教授 (80359794)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | リキッドバイオプシー / バイオマーカー / 血漿DNA / EGFR T790M / plasma DNA / metastasis / mouse model / ＥＧＦＲ Ｔ７９０Ｍ / ｐｌａｓｍａ ＤＮＡ / ｍｅｔａｓｔａｓｉｓ / ｍｏｕｓｅ ｍｏｄｅｌ |
|---|
| Outline of Final Research Achievements |
We established a highly metastatic animal model using immunodeficient mice inoculated with human lung cancer cell line, H1975 carrying EGFR mutations. Tumor-derived circulating DNA (ctDNA) was analyzed using MBP-QP method on the animal model, and the results are obtained as follows:1) Frequency of ctDNA detection was correlated with tumor burden and metastasis, suggesting that ctDNA appears in peripheral blood concomitant with tumor progression.2) Organ preference of metastasis was analyzed. Inoculation with H1975 and H226B was metastasized into lymph nodes and lung, respectively. Several molecules were more phosphorylated in metastatic lymph nodes compared to primary lesion.3) Monitoring of anti-tumor effect of 3rd generation EGFR tyrosine kinase inhibitor could be performed by detection of ctDNA.
|
