Analysis how circulating plasma DNA release to peripheral blood and that applies to metastatic animal model using human lung cancer cells
Project/Area Number |
26430144
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor diagnostics
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Research Institution | Saga University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
佐藤 明美 佐賀大学, 医学部, 助教 (20568357)
中村 朝美 佐賀大学, 医学部, 助教 (90457490)
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Co-Investigator(Renkei-kenkyūsha) |
KIMURA SHINYA 佐賀大学, 医学部, 教授 (80359794)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | リキッドバイオプシー / バイオマーカー / 血漿DNA / EGFR T790M / plasma DNA / metastasis / mouse model / EGFR T790M / plasma DNA / metastasis / mouse model |
Outline of Final Research Achievements |
We established a highly metastatic animal model using immunodeficient mice inoculated with human lung cancer cell line, H1975 carrying EGFR mutations. Tumor-derived circulating DNA (ctDNA) was analyzed using MBP-QP method on the animal model, and the results are obtained as follows:1) Frequency of ctDNA detection was correlated with tumor burden and metastasis, suggesting that ctDNA appears in peripheral blood concomitant with tumor progression.2) Organ preference of metastasis was analyzed. Inoculation with H1975 and H226B was metastasized into lymph nodes and lung, respectively. Several molecules were more phosphorylated in metastatic lymph nodes compared to primary lesion.3) Monitoring of anti-tumor effect of 3rd generation EGFR tyrosine kinase inhibitor could be performed by detection of ctDNA.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Detection of KRAS Mutations in plasma DNA using a fully automated rapid detection system in colorectal cancer patients2017
Author(s)
Hosoya K, Matsusaka S, Kashiwada T, Suzuki K, Ureshino N, Sato A, Miki Y, Kitera K, Hirai M, Hatake K, Kimura S, Sueoka-Aragane N
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Journal Title
Pathol Oncol Res
Volume: Epub ahead of print
Pages: 1007-1007
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Monitoring EGFR T790M with plasma DNA from lung cancer patients in a prospective observational study2016
Author(s)
Sueoka-Aragane N, Katakami N, Satouchi M, Yokota S, Aoe K, Iwanaga K, Otsuka K, Morita S, Kimura S, Negoro S, For Hanshin-Saga Collaborative Cancer Study Group
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Journal Title
Cancer Sci
Volume: 107
Pages: 162-167
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Non-invasive monitoring system for acquired resistance to EGFR-tyrosine kinase inhibitors using circulating plasma DNA2014
Author(s)
Naoko Sueoka-Aragane, Tomomi Nakamura, Akemi Sato, Naomi Kobayashi, Kentaro Iwanaga, Kazutoshi Komiya, Toshiya Hosomi, Mitsuharu Hirai, Eisaburo Sueoka, Seiji Okada, Shinya Kimura
Organizer
73th Annual Meeting of the Japanese Cancer Association
Place of Presentation
横浜市
Year and Date
2014-09-25 – 2014-09-27
Related Report
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[Presentation] Monitoring of metastasis by detection of EGFR mutation, T790M, with plasma using animal model for metastasis of human lung cancer2014
Author(s)
Naoko Sueoka-Aragane, Nobuyuki Katakami, Miyako Satouchi, Soichiro Yokota, Keisuke Aoe, Kentaro Iwanaga, Kojiro Otsuka, Shinya Kimura, Shunichi Negoro
Organizer
2014 ASCO Annual Meeting
Place of Presentation
米国シカゴ
Year and Date
2014-05-30 – 2014-06-03
Related Report
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