Genomic instability of ALK fusion positive lung adenocarcinoma

• Project/Area Number: 26430149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: NINOMIYA HIRONORI 公益財団法人がん研究会, がん研究所 病理部, 研究員 (20462228)
• Co-Investigator(Kenkyū-buntansha): 石川 雄一 公益財団法人がん研究会, がん研究所 病理部, 部長 (80222975)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肺腺癌 / ALK融合遺伝子 / ゲノム不安定性 / クロモトリプシス / chromothripsis / 遺伝子増幅 / EML4-ALK陽性 / 腺扁平上皮癌 / EML4-ALK / CGHアレイ

Outline of Final Research Achievements

We analyzed surgically resected pulmonary adenocarcinomas with Copy number analyzer for GeneChip (CNAG). The result revealed that frequent copy number change occurred on the short arm of chromosome 2 among ALK fusion positive cases. Same genomic alteration patter was detected in 10 cases among 35 (28.5%). On the other hand, no case showed similar copy number change among ALK fusion negative cases. The result revealed that genomic fragmentation and reconstruction is a notable mechanism of ALK fusion gene formation leading to carcinogenesis.

Research Products

• [Presentation] 肺癌のゲノムワイド解析 (2018)
  • Author(s): 稲村健太郎
  • Organizer: 日本病理学会総会 札幌 6月21日-6月23日
• [Presentation] EML4-ALK陽性腺扁平上皮癌の2例 (2016)
  • Author(s): 二宮 浩範
  • Organizer: 第57回日本肺癌学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡サンパレス、福岡国際センター
  • Year and Date: 2016-12-19
• [Presentation] EML4-ALK陽性扁平上皮癌成分を有する２症例の分子生物学的解析 (2016)
  • Author(s): 二宮 浩範
  • Organizer: 第75回日本癌学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06
• [Presentation] Unique pattern of copy number changes including chromothripsis in pulmonary adenocarcinoma with EML4-ALK fusion (2015)
  • Author(s): Hironori Ninomiya
  • Organizer: AACR annual meeting 2015
  • Place of Presentation: Pennsylvania Convention Center Philadelphia, Pennsylvania.
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] ALK肺腺癌において高悪性度組織亜型Solid，Micropapは予後因子となるか (2014)
  • Author(s): 松浦陽介, 元井紀子, 二宮浩範, 山崎宏嗣, 平井慶充, 後藤英典, 森彰平, 鮫島譲司, 中尾将之, 上原浩文, 文敏景, 中川健, 石川雄一, 奥村栄
  • Organizer: 第55回日本肺癌学会
  • Place of Presentation: 京都
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] Lung adenocarcinoma with unique pathological features (2014)
  • Author(s): Hironori Ninomiya, Yuichi Ishikawa
  • Organizer: IAP日台スライドカンファレンス
  • Place of Presentation: 台湾 台北
  • Year and Date: 2014-10-25 – 2014-10-26
• [Funded Workshop] AACR annual meeting (2015)
  • Place of Presentation: Pennsylvania Convention Center
  • Year and Date: 2015-04-18