| Project/Area Number |
26430150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnostics
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| Research Institution | Shizuoka Cancer Center Research Institute |
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Principal Investigator |
Ohshima Keiichi 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (10399587)
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| Co-Investigator(Kenkyū-buntansha) |
寺島 雅典 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (40197794)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腫瘍マーカー / プロホルモン変換酵素 / ELISA / セクレトーム / 培養細胞 / プロテオーム / エクソーム / トランスクリプトーム / プロテオミクス |
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| Outline of Final Research Achievements |
Prohormone is a protein precursor to produce a bioactive peptide by processing, in which prohormone convertases (PCs) are involved. Prohormone of gastric releasing peptide (GRP), proGRP, is abundant and stable in blood from small cell lung cancer patients, resulting in development of a tumor marker. The aim of this study is to identify a novel prohormone-derived tumor marker by estimating the processing function of PCs followed by proteomics-based identification of secreted prohormones. Using cancer cell lines derived from various tissues, including lung, stomach, and colon, along with clinical specimens of tumor and matched normal tissue and blood samples, we profiled gene expression, mutation status, and copy number change in 9 PC genes. This dataset is an invaluable resource for further identification of prohormones in the extracellular spaces. Using the dataset profiling all genes, we also clarified genes with amplification-dependent overexpression to predict cancer driver genes.
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