Exploration and evaluation of carbohydrate tumor marker

• Project/Area Number: 26430151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: MIYAMOTO Yasuhide 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, 総括研究員(分子生物学部門長) (90322742)
• Research Collaborator: Okamoto Miki 地方独立行政法人大阪府立病院機構, 大阪府立成人病センター(研究所)・その他の部局, 研究員 (20332455)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糖鎖 / 腫瘍マーカー / 癌

Outline of Final Research Achievements

We have attempted to identify a novel glycan tumor marker by comparing the serum O-glycan profiles from healthy controls with those of cancer patients. We identified 3 marker candidates. The precise oligosaccharide structures of these candidates was clarified. To quantify these glycans, we developed the selected reaction monitoring (SRM) assay, which used a stable isotope-labeled glycan as internal standard with a high level of sensitivity and accuracy. Verification study of one of the candidate was then performed by SRM analyses.

Research Products

• [Journal Article] Cytoplasmic calcium increase via fusion with inactivated Sendai virus induces apoptosis in human multiple myeloma cells by downregulation of c-Myc oncogene. (2016)
  • Author(s): Jiang Y, Saga K, Miyamoto Y, Kaneda Y.
  • Journal Title: Oncotarget Volume: 7 Issue: 24 Pages: 36034-36048
  • DOI: 10.18632/oncotarget.9105
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Elevation of CA19-9-Related Novel Marker, Core 1 Sialyl Lewis A, in Sera of Adenocarcinoma Patients Verified by a SRM-Based Method. (2016)
  • Author(s): Tanaka-Okamoto M, Yabu M, Mukai M, Takahashi H, Fujiwara Y, Ohue M, Kamada Y, Miyoshi E, Miyamoto Y.
  • Journal Title: Journal of Proteome Research Volume: 15 Issue: 1 Pages: 152-165
  • DOI: 10.1021/acs.jproteome.5b00893
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Precise structural analysis of O-linked oligosaccharides in human serum. (2014)
  • Author(s): Yabu, M., Korekane, H., Miyamoto, Y.
  • Journal Title: Glycobiology Volume: 24(6) Issue: 6 Pages: 542-553
  • DOI: 10.1093/glycob/cwu022
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] フォーカストグライコミクスによって同定された硫酸付加された糖鎖腫湯マーカー候補群 (2016)
  • Author(s): 岡本三紀、宮本泰豪,
  • Organizer: 第34回日本糖質学会
  • Place of Presentation: 高知市文化プラザ(高知)
  • Year and Date: 2016-09-01
• [Presentation] 1.SRM法を用いた血清糖鎖腫瘍マーカー候補core 1 sialyl Lewis Aの定量解析 (2015)
  • Author(s): 宮本泰豪、岡本三紀、高橋秀典、向井幹夫、藤原義之、大植雅之
  • Organizer: 第74回日本癌学会学術集会,
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2015-10-08
• [Presentation] SRM法を用いた血清糖鎖腫瘍マーカー候補 core 1 sialyl Lewis Aの定量解析 (2015)
  • Author(s): 岡本三紀、藪政彦、宮本泰豪
  • Organizer: 第34回日本糖質学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2015-07-31
• [Presentation] 藪政彦、岡本三紀、是金宏昭、宮本泰豪ヒト血清中のO型糖鎖の詳細な構造解析 (2014)
  • Author(s): 藪政彦、岡本三紀、是金宏昭、宮本泰豪
  • Organizer: 第33回日本糖質学会
  • Place of Presentation: 名古屋大学豊田講堂(名古屋)
  • Year and Date: 2014-08-10 – 2014-08-12