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Development of oncolytic RNA virus sensitive to ganciclovir

Research Project

Project/Area Number 26430155
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionChiba University

Principal Investigator

SHIRASAWA Hiroshi  千葉大学, 大学院医学研究院, 教授 (00216194)

Co-Investigator(Kenkyū-buntansha) 齋藤 謙悟  千葉大学, 大学院医学研究院, 講師 (70451755)
菅波 晃子  千葉大学, 大学院医学研究院, 助教 (10527922)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords腫瘍融解ウイルス / ガンシクロビル / RNAウイルス / HSV tk
Outline of Final Research Achievements

An RNA virus, SIN, is an oncolytic virus, which cause no integration of viral genome into chromosome, specific infection and proliferation in cancer cells. In this study, to improve the safety of SIN as an oncolytic virus, we developed a SIN that expresses herpesvirus tk gene and is sensitive to anti-herpesvirus drug ganciclovir (GCV).
A recombinant SIN, SINtkGFPmE2, which have chimera gene of tk and GFP between capsid gene (C) and envelope protein gene (E2) mutated in 1 amino acid, was the most stable among recombinants we constructed. The SINtkGFPmE2 was sensitive to GCV and the proliferation was inhibited.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (23 results)

All 2016 2015 2014

All Journal Article (17 results) (of which Peer Reviewed: 17 results,  Open Access: 17 results,  Acknowledgement Compliant: 5 results) Presentation (6 results)

  • [Journal Article] Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB2016

    • Author(s)
      Wu S, Kanda T, Nakamoto S, Jiang X, Nakamura M, Sasaki R, Haga Y, Shirasawa H, Yokosuka O.
    • Journal Title

      Int J Mol Med.

      Volume: 38 Issue: 2 Pages: 475-481

    • DOI

      10.3892/ijmm.2016.2643

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hepatitis C virus genotypes in Myanmar2016

    • Author(s)
      Win NN, Kanda T, Nakamoto S, Yokosuka O, Shirasawa H.
    • Journal Title

      World J Gastroenterol.

      Volume: 22 Issue: 27 Pages: 6095-6099

    • DOI

      10.3748/wjg.v22.i27.6095

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility of viral dust infection via air movement and dispersion of dried viral particles from the floor2016

    • Author(s)
      Mori K, Onuki A, Kanou F, Akiba T, Hayashi Y, Shirasawa H, Sadamasu K.
    • Journal Title

      J Med Virol.

      Volume: 89 Issue: 5 Pages: 931-935

    • DOI

      10.1002/jmv.24710

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Free fatty acids or high-concentration glucose enhances hepatitis A virus replication in association with a reduction in glucose-regulated protein 78 expression2016

    • Author(s)
      Nan Nwe Win, Tatsuo Kanda, Masato Nakamura, Shingo Nakamoto, Hiroaki Okamoto, Osamu Yokosuka, Hiroshi Shirasawa
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: S0006-291X Issue: 1 Pages: 32124-32126

    • DOI

      10.1016/j.bbrc.2016.12.080

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Possible Involvement of Hepatitis B Virus Infection of Hepatocytes in the Attenuation of Apoptosis in Hepatic Stellate Cells.2016

    • Author(s)
      Sasaki R, Kanda T, Nakamura M, Nakamoto S, Haga Y, Wu S, Shirasawa H, Yokosuka O.
    • Journal Title

      PLoS One.

      Volume: 11 Issue: 1 Pages: 1-17

    • DOI

      10.1371/journal.pone.0146314

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Liposomally formulated phospholipid-conjugated indocyanine green for intra-operative brain tumor detection and resection.2015

    • Author(s)
      Suganami A, Iwadate Y, Shibata S, Yamashita M, Tanaka T, Shinozaki N, Aoki I, Saeki N, Shirasawa H, Okamoto Y, Tamura Y.
    • Journal Title

      Int J Pharm.

      Volume: 496 Issue: 2 Pages: 401-406

    • DOI

      10.1016/j.ijpharm.2015.10.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The sirtuin inhibitor sirtinol inhibits hepatitis A virus (HAV) replication by inhibiting HAV internal ribosomal entry site activity.2015

    • Author(s)
      Kanda T, Sasaki R, Nakamoto S, Haga Y, Nakamura M, Shirasawa H, Okamoto H, Yokosuka O.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 466 Issue: 3 Pages: 567-571

    • DOI

      10.1016/j.bbrc.2015.09.083

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Hepatitis C Virus Genotype 1b Core and NS5A Mutations on Response to Peginterferon Plus Ribavirin Combination Therapy.2015

    • Author(s)
      Nakamoto S, Imazeki F, Arai M, Yasui S, Nakamura M, Haga Y, Sasaki R, Kanda T, Shirasawa H, Yokosuka O.
    • Journal Title

      Int J Mol Sci.

      Volume: 16 Issue: 9 Pages: 21177-21190

    • DOI

      10.3390/ijms160921177

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oncolytic viral therapy for neuroblastoma cells with Sindbis virus AR339 strain.2015

    • Author(s)
      Takenouchi A, Saito K, Saito E, Saito T, Hishiki T, Matsunaga T, Isegawa N, Yoshida H, Ohnuma N, Shirasawa H.
    • Journal Title

      Pediatr Surg Int.

      Volume: 31 Issue: 12 Pages: 1151-1159

    • DOI

      10.1007/s00383-015-3784-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association between hepatitis B virus and MHC class I polypeptide-related chain A in human hepatocytes derived from human-mouse chimeric mouse liver.2015

    • Author(s)
      Sasaki R, Kanda T, Wu S, Nakamoto S, Haga Y, Jiang X, Nakamura M, Shirasawa H, Yokosuka O.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 464 Issue: 4 Pages: 1192-1195

    • DOI

      10.1016/j.bbrc.2015.07.102

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Antiviral therapies for chronic hepatitis C virus infection with cirrhosis.2015

    • Author(s)
      Nakamoto S, Kanda T, Shirasawa H, Yokosuka O.
    • Journal Title

      World J Hepatol.

      Volume: 7 Issue: 8 Pages: 1133-1141

    • DOI

      10.4254/wjh.v7.i8.1133

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alteration of cell cycle progression by Sindbis virus infection.2015

    • Author(s)
      Yi R, Saito K, Isegawa N, Shirasawa H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 462 Issue: 4 Pages: 426-432

    • DOI

      10.1016/j.bbrc.2015.04.148

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The JAK2 inhibitor AZD1480 inhibits hepatitis A virus replication in Huh7 cells.2015

    • Author(s)
      Jiang X, Kanda T, Nakamoto S, Saito K, Nakamura M, Wu S, Haga Y, Sasaki R, Sakamoto N, Shirasawa H, Okamoto H, Yokosuka O
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 印刷中 Issue: 4 Pages: 908-912

    • DOI

      10.1016/j.bbrc.2015.02.058

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reactivation of Hepatitis B Virus in Hematopoietic Stem Cell Transplant Recipients in Japan: Efficacy of Nucleos(t)ide Analogues for Prevention and Treatment.2014

    • Author(s)
      Nakamoto S, Kanda T, Nakaseko C, Sakaida E, Ohwada C, Takeuchi M, Takeda Y, Mimura N, Iseki T, Wu S, Arai M, Imazeki F, Saito K, Shirasawa H, Yokosuka O.
    • Journal Title

      Int J Mol Sci.

      Volume: 15 Pages: 21455-21467

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Hepatitis C Virus Nonstructural Protein 5A Inhibits Thapsigargin-Induced Apoptosis2014

    • Author(s)
      Xia Jiang, Tatsuo Kanda, Shuang Wu, Shingo Nakamoto, Takaji Wakita, Hiroshi Shirasawa, Osamu Yokosuka.
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 11 Pages: e113499-e113499

    • DOI

      10.1371/journal.pone.0113499

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Suppression of La Antigen Exerts Potential Antiviral Effects against Hepatitis A Virus.2014

    • Author(s)
      Jiang X, Kanda T, Wu S, Nakamoto S, Saito K, Shirasawa H, Kiyohara T, Ishii K, Wakita T, Okamoto H, Yokosuka O.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 7 Pages: e101993-e101993

    • DOI

      10.1371/journal.pone.0101993

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] , Hepatitis C virus NS5A inhibitors and drug resistance mutations.2014

    • Author(s)
      Nakamoto S, Kanda T, Wu S, Shirasawa H, Yokosuka O.
    • Journal Title

      World J Gastroenterol

      Volume: 20 Pages: 2902-2912

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ベンゾイミダゾール誘導体 C299-1 による癌細胞傷害機序の解析2016

    • Author(s)
      大野 吉史、蟻 瑞栄、陳 偉巍、田 錚、郭 書翰、李 斉森、馬 雪、菅波 晃子、田村 裕、松本 明郎、松本 祥治、齋藤 謙悟、白澤 浩
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規化合物 CCL-113 は M 期とエンドサイクルを誘導する2016

    • Author(s)
      坪坂 歩、蟻 瑞栄、大野 吉史、陳 偉巍、田 錚、郭 書翰、李 斉森、馬 雪、菅波 晃子、齋藤 謙悟、田村 裕、白澤 浩
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Alteration of cell cycle progression by Sindbis virus infection2016

    • Author(s)
      Yoshifumi Ohno, Yi Ruirong, Chen Weiwei, Tian Zheng, Guo Shuhan, Qisen Li, Ma Xue, Akiko Suganami, Kengo Saito, Yutaka Tamura, Naohisa Isegawa, Hiroshi Shirasawa
    • Organizer
      The 64th annual meeting of the Japanese society for virology
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Screening and identification of compounds with antiviral activity against human respiratory syncytial virus.2015

    • Author(s)
      Yoshifumi Ohno, Yi Ruirong, Chen Weiwei, Tian Zheng, Guo Shuhan, Ma Xue, Akiko Suganami, Kengo Saito, Yutaka Tamura, Hiroshi Shirasawa
    • Organizer
      The 63rd annual meeting of the Japansese society for virology
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 悪性黒色腫治療へのLP-iDOPEの適用2015

    • Author(s)
      菅波 晃子、篠田 健太、鈴木 茜、齋藤 謙悟、岡本 芳晴、本橋 新一郎、中山 俊憲、白澤 浩、田村 裕
    • Organizer
      第74回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 腫瘍溶解ウイルスSindbis virusは癌細胞をS期に誘導する2015

    • Author(s)
      大野 吉史、蟻 瑞栄、陳 偉巍、田 錚、郭 書翰、馬 雪、菅波 晃子、齋藤 謙悟、伊勢川 直久、田村 裕、白澤 浩
    • Organizer
      第74回日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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