Development of novel anti-tumor therapy target mRNA cleavage using a new method

• Project/Area Number: 26430157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Niigata University
• Principal Investigator: Miwako Narita 新潟大学, 医歯学系, 教授 (30281009)
• Co-Investigator(Kenkyū-buntansha): 梨本 正之 新潟薬科大学, 健康・自立総合研究機構, 教授 (30228069)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: small guide RNA / TRUE gene silencing / 白血病 / sgRNA / tRNase Z / apoptosis / NB4 / ヘプタマーsgRNA / 腫瘍 / 腫瘍細胞 / HL-60 / WT1

Outline of Final Research Achievements

In order to use the TRUE gene silence method using sgRNA for treatment of hematologic malignancies, sgRNA was prepared using various tumor - associated mRNA as a target, and suppression of cell proliferation and apoptosis induction were examined. In several leukemia cell lines, the effect of sgRNA on WT1 and Bcl-2 was confirmed. It was also confirmed that this effect varied depending on the concentration of glucose in the culture medium.We could establish condition setting to predict the effect of sgRNA.

Research Products

• [Journal Article] Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies. (2014)
  • Author(s): Takahashi M, Elbarbary RA, Watanabe N, Goto A, Kamiya D, Watabe Y, Uchiyama T, Narita M, Takahashi M, Takahashi Y, Ishihara N, Miyazawa T, Yoshida T, Kawano M, Tamura M, Nashimoto M.
  • Journal Title: Leuk Research Volume: 38 Pages: 808-815
  • Peer Reviewed
• [Patent(Industrial Property Rights)] United States Patent (2014)
  • Inventor(s): M Nashimoto, M Narita,他
  • Industrial Property Rights Holder: M Nashimoto, M Narita,他
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014
  • Acquisition Date: 2017
  • Overseas