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Development of novel anti-tumor therapy target mRNA cleavage using a new method

Research Project

Project/Area Number 26430157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionNiigata University

Principal Investigator

Miwako Narita  新潟大学, 医歯学系, 教授 (30281009)

Co-Investigator(Kenkyū-buntansha) 梨本 正之  新潟薬科大学, 健康・自立総合研究機構, 教授 (30228069)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordssmall guide RNA / TRUE gene silencing / 白血病 / sgRNA / tRNase Z / apoptosis / NB4 / ヘプタマーsgRNA / 腫瘍 / 腫瘍細胞 / HL-60 / WT1
Outline of Final Research Achievements

In order to use the TRUE gene silence method using sgRNA for treatment of hematologic malignancies, sgRNA was prepared using various tumor - associated mRNA as a target, and suppression of cell proliferation and apoptosis induction were examined. In several leukemia cell lines, the effect of sgRNA on WT1 and Bcl-2 was confirmed. It was also confirmed that this effect varied depending on the concentration of glucose in the culture medium.We could establish condition setting to predict the effect of sgRNA.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies.2014

    • Author(s)
      Takahashi M, Elbarbary RA, Watanabe N, Goto A, Kamiya D, Watabe Y, Uchiyama T, Narita M, Takahashi M, Takahashi Y, Ishihara N, Miyazawa T, Yoshida T, Kawano M, Tamura M, Nashimoto M.
    • Journal Title

      Leuk Research

      Volume: 38 Pages: 808-815

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Patent(Industrial Property Rights)] United States Patent2014

    • Inventor(s)
      M Nashimoto, M Narita,他
    • Industrial Property Rights Holder
      M Nashimoto, M Narita,他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014
    • Acquisition Date
      2017
    • Related Report
      2017 Annual Research Report
    • Overseas

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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