ChIP-Injection: Identification of functional enhancers using zebrafish embryos
| Project/Area Number |
26430182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エンハンサー / スクリーニング / クロマチン免疫沈降法 / ゼブラフィッシュ / ヒストン修飾 |
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| Outline of Final Research Achievements |
Using zebrafish embryos, we established a ChIP-Injection method that enables identification of functional enhancers based on their enhancer activities in embryos. Each reporter gene possessing the enhancer-associated genomic region enriched by ChIP was injected into zebrafish embryos to analyze the activity of putative enhancers. By using the ChIP-Injection, we identified 32 distinct putative enhancers that drove specific expression. Additionally, we generated transgenic lines that exhibit distributions of the EGFP signal as was observed in the screening. Furthermore, the expression pattern driven by the identified somite-specific enhancer resembled that of the gene acta2. The results indicate that ChIP-Injection provides an efficient approach for identification of active enhancers in a potentially wide variety of developmental tissues and stages.
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Report
(5 results)
Research Products
(42 results)
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[Journal Article] Posterior-anterior gradient of zebrafish hes6 expression in the presomitic mesoderm is established by the combinatorial functions of the downstream enhancer and 3'UTR.2016
Author(s)
(14)Kawamura, A., Ovara, H., Ooka, Y., Kinoshita, H., Hoshikawa, M., Nakajo, K., Yokota, D., Fujino, Y., Higashijima, S., Takada, S., Yamasu, K.
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Journal Title
Developmental Biology
Volume: 409
Pages: 543-554
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] ゼブラフィッシュ未分節中胚葉におけるtbx6遺伝子の転写制御機構の解析2018
Author(s)
伴 博之, 横田 大佑, 乙坂 栞里, 木下 宏史, 藤野 友梨, 矢部 泰二郎, 小原 弘幹, 猪塚 彩花, 赤間 燿, 鹿毛 大地, 弥益 恭, 高田 慎治, 川村 哲規
Organizer
第70回 日本動物学会 関東支部大会
Related Report
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[Presentation] Genetic interactions between Ripply and Tbx6 in the somite segmentation and myogenesis in zebrafish embryos2017
Author(s)
Kinoshita H, Fujino Y, Ohgane N, Yabe T, Yokota D, Ovara H, Izuka A, Ban H, Kage D, Yamasu K, Takada S, and Kawamura A
Organizer
50th Annual Meeting of the Japanese Society of Developmental Biologists
Related Report
Int'l Joint Research
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[Presentation] Transcriptional autoregulatory loop maintains the expression of tbx6 in the zebrafish presomitic mesoderm2017
Author(s)
Ban H, Yokota D, Kinoshita H, Fujino Y, Yabe T, Ovara H, Izuka A, Akama K, Kage D, Yamasu K, Takada S, and Kawamura A
Organizer
The 23rd Japanese Medaka and Zebrafish Meeting
Related Report
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[Presentation] Relative levels of ripply and tbx6 are required for the somite segmentation in zebrafish2016
Author(s)
Kinoshita H, Ohgane N, Fujino Y, Yabe T, Yokota D, Ovara H, Izuka A, Kage D, Yamasu K, Takada S, and Kawamura A
Organizer
The 22nd International Congress of Zoology & The 87th meeting of Zoological Society of Japan
Place of Presentation
沖縄コンベンションセンター（沖縄県・宜野湾市）
Year and Date
2016-11-15
Related Report
Int'l Joint Research
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