Functional analysis of Peg10, a paternally expressed imprinted gene

• Project/Area Number: 26430183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Ono Ryuichi 国立医薬品食品衛生研究所, 毒性部, 室長 (10401358)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 胎盤 / レトロトランスポゾン

Outline of Final Research Achievements

We have produced Peg10 mutant mice by introducing DSB using CRISPR/Cas9 system to analyze detailed function of Peg10. Then, we found that about 30 % of mutant mice had long de novo retrotransposon insertion(s) at DSB-introduced sites. In other cases, mutant mice had insertions from spliced mRNAs , indicating the involvement of reverse transcription (RT).In conclusion, RT-product, in the mouse zygote, were captured at DSB loci mediated by micro homology.

Research Products

• [Journal Article] Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes. (2015)
  • Author(s): Ono R., Ishii M., Fujihara Y., Kitazawa M., Usami T., Kaneko-Ishino T., Kanno J., Ikawa M., Ishino F.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 12281-12281
  • DOI: 10.1038/srep12281
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cognitive Function Related to the Sirh11/Zcchc16 Gene Acquired from an LTR Retrotransposon in Eutherians. (2015)
  • Author(s): Irie M., Yoshikawa M., Ono R., Iwafune H., Furuse T., Yamada I., Wakana S., Yamashita Y., Abe T., *Ishino F., Kaneko-Ishino T.
  • Journal Title: PLoS Genetics Volume: 11 Issue: 9 Pages: 1005521-1005521
  • DOI: 10.1371/journal.pgen.1005521
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sirh7/Ldoc1 knockout mice exhibit placental P4 overproduction and delayed parturition. (2014)
  • Author(s): Naruse M, Ono R, Irie M, Nakamura K, Furuse T, Hino T, Oda K, Kashimura M, Yamada I, Wakana S, Yokoyama M, Ishino F, Kaneko-Ishino T.
  • Journal Title: Development Volume: 141 Issue: 24 Pages: 4763-4771
  • DOI: 10.1242/dev.114520
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Double strand break repair by capture of unintentional sequences, an emerging new risk for the leading-edge technology. (2017)
  • Author(s): Ryuichi Ono
  • Organizer: Keystone Symposia Conference / Precision Genome Engineering
  • Place of Presentation: Breckenridge, Colorado USA
  • Year and Date: 2017-01-08
  • Int'l Joint Research
• [Presentation] Double strand break repair by capture of retrotransposon and mRNA sequences via reverse transcription in the mouse zygote. (2015)
  • Author(s): Ono, R.
  • Organizer: FASEB "Mobile DNA in Mammalian Genomes"
  • Place of Presentation: Palm Beach, USA
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Presentation] レトロトランスポゾン獲得による哺乳類の胎生進化 (2014)
  • Author(s): 小野竜一
  • Organizer: 第17回 SUMMER RETROVIRUS CONFERENCE
  • Place of Presentation: 伊豆山研修センター
  • Year and Date: 2014-07-03 – 2014-07-05
  • Invited