Functional analysis of Peg10, a paternally expressed imprinted gene
Project/Area Number |
26430183
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | National Institute of Health Sciences |
Principal Investigator |
Ono Ryuichi 国立医薬品食品衛生研究所, 毒性部, 室長 (10401358)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 胎盤 / レトロトランスポゾン |
Outline of Final Research Achievements |
We have produced Peg10 mutant mice by introducing DSB using CRISPR/Cas9 system to analyze detailed function of Peg10. Then, we found that about 30 % of mutant mice had long de novo retrotransposon insertion(s) at DSB-introduced sites. In other cases, mutant mice had insertions from spliced mRNAs , indicating the involvement of reverse transcription (RT).In conclusion, RT-product, in the mouse zygote, were captured at DSB loci mediated by micro homology.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Cognitive Function Related to the Sirh11/Zcchc16 Gene Acquired from an LTR Retrotransposon in Eutherians.2015
Author(s)
Irie M., Yoshikawa M., Ono R., Iwafune H., Furuse T., Yamada I., Wakana S., Yamashita Y., Abe T., *Ishino F., Kaneko-Ishino T.
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Journal Title
PLoS Genetics
Volume: 11
Issue: 9
Pages: 1005521-1005521
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Sirh7/Ldoc1 knockout mice exhibit placental P4 overproduction and delayed parturition.2014
Author(s)
Naruse M, Ono R, Irie M, Nakamura K, Furuse T, Hino T, Oda K, Kashimura M, Yamada I, Wakana S, Yokoyama M, Ishino F, Kaneko-Ishino T.
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Journal Title
Development
Volume: 141
Issue: 24
Pages: 4763-4771
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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