| Project/Area Number |
26430188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石原 直恵 (琴村直恵 / 石原 直恵(琴村直恵)) 藤田保健衛生大学, 医学部, 研究員 (50571791)
原田 信広 藤田保健衛生大学, 医学部, 教授 (00189705)
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| Co-Investigator(Renkei-kenkyūsha) |
NIKAIDO ITOSHI 独立行政法人理化学研究所, 情報基盤センター, ユニットリーダー (00383290)
SASAGAWA YOHEI 独立行政法人理化学研究所, 情報基盤センター, 上級センター研究員 (10404344)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | クロマチン / ヌクレオソーム / SEVENS法 / 転写 / プロモーター / CYP19 / 次世代シーケンサー / セントロメア / ヒストン修飾 |
|---|
| Outline of Final Research Achievements |
A controlled amount of RNA is transcribed from an individual gene. To elucidate contribution of chromatin structure to this quantitative control, the CYP19 gene, which harbors three promoters with a different activity, was chosen as a model gene. The SEVENS assay revealed that local density of nucleosomes in the promoter regions was inversely correlated to transcriptional activity of each promoter of the CYP19 gene. Such correlation was confirmed by a whole genome analysis in which DNA recovered from the SEVENS fractions was sequenced using next generation sequencing technology.
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