Genome research elucidating environmental adaptation of Dark-fly
| Project/Area Number |
26430189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Tohoku University (2016)
Kyoto University (2015)
The Institute of Physical and Chemical Research (2014) |
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Principal Investigator |
Fuse Naoyuki 東北大学, 薬学研究科, 助教 (80321983)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 環境適応 / ショウジョウバエ / ゲノム / トランスクリプトーム / ゲノム編集 / ロドプシン / 光 |
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| Outline of Final Research Achievements |
Organisms are remarkably adapted to diverse environments, but molecular mechanism underlying environmental adaptation still remains elusive. To address this issue, we utilize a unique Drosophila line, "Dark-fly", which has been maintained for 62 years in a constant dark condition.
We determined whole genome sequence of Dark-fly using next-generation sequencer and identified approximately 220,000 single nucleotide polymorphisms (SNPs) in the Dark-fly genome. To identify SNPs associated with dark-adaptation, we reared mixed populations of Dark-fly and the wild type fly in light and dark conditions. Our analyses of population genome detected environment-dependent selections of the Dark-fly genome. We consequently listed 84 candidate genes potentially involved in the adaptation of Dark-fly. We currently aim to clarify the roles of Dark-fly's SNPs in environmental adaptation using genome editing technology.
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Report
(4 results)
Research Products
(3 results)
