Genome Editing to Improve Abiotic Stress Responses in Plants
Project/Area Number |
26430190
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | The University of Tokushima (2015-2016) The Institute of Physical and Chemical Research (2014) |
Principal Investigator |
Osakabe Yuriko 徳島大学, 大学院生物資源産業学研究部(連携), 特任准教授 (50444071)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | ゲノム編集 / 環境ストレス / イオン輸送体 / ストレス応答 / CRISPR/CAS9 |
Outline of Final Research Achievements |
Genome editing is now widely utilized to modify various plant genomes. Using a truncated gRNA (tru-gRNA)/Cas9, we generated new alleles for OST2, a proton pump in Arabidopsis, with no off-target effects. Newly generated mutations in CRIPSR/Cas9 transgenic plants were detected with the average mutation rates of 32.8% using a constitutive promoter for Cas9 expression. The reducing nuclear localization signals (NLS) in Cas9 decreased the mutation rates. The tru-gRNA/Cas9 driven by the meristematic- and reproductive tissue-specific promoters increased the heritable mutation rate in Arabidopsis, showing that high expression in the germ line can produce bi-allelic mutations with high mutation efficiency. Finally, we generated the new mutant alleles for OST2, and the mutants exhibited altered stomatal closing in response to stress conditions. The results suggest further applications in molecular breeding to improve plant function using optimized CRISPR/Cas9.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Positive regulatory role of strigolactone in plant responses to drought and salt stress.2014
Author(s)
Ha CV, Leyva-Gonzalez MA, Osakabe Y, Tran UT, Nishiyama R, Watanabe Y, Tanaka M, Seki M, Yamaguchi S, Dong NV, Yamaguchi-Shinozaki K, Shinozaki K, Herrera-Estrella L, Tran LS.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 111(2)
Issue: 2
Pages: 851-856
DOI
Related Report
Peer Reviewed
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[Journal Article] Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits2014
Author(s)
Hikaru Sato, Junya Mizoi, Hidenori Tanaka, Kyonosin Maruyama, Feng Qin, Yuriko Osakabe, Kyoko Morimoto, Teppei Ohori, Kazuya Kusakabe, Maika Nagata, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
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Journal Title
Plant Cell
Volume: 26
Issue: 12
Pages: 4954-4973
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] リンゴゲノムの多様性とゲノム編集による改変2016
Author(s)
西谷 千佳子, 平井 徳美, 小森 貞男, 和田 雅人, 岡田 和馬, 刑部 敬史, 山本 俊哉, 刑部 祐里子
Organizer
日本植物学会第80回大会シンポジウム
Place of Presentation
沖縄コンベンションセンター(沖縄県沖縄市)
Year and Date
2016-09-16
Related Report
Invited
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[Presentation] ゲノム編集技術による植物環境応答能の改変2016
Author(s)
刑部 祐里子, 渡辺 崇人, 菅野 茂夫, 上田 梨紗, 石原 諒典, 篠崎 一雄, 刑部 敬史
Organizer
日本ゲノム編集学会第1回大会
Place of Presentation
広島国際会議場(広島県広島市)
Year and Date
2016-09-06
Related Report
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