| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Using the RIKEN NPDepo chemical library, we try to identify the chemical compounds which suppress the damage of photosynthesis under heat stress by using chlorophyll fluorescence as an indicator. In first screening, we used the 80 of Authentic Library which contains standard natural compounds with bioactivity. Preliminary data show that chemical screening is useful for isolation of novel compounds that affect heat stress. In second screening that used the 400 of Pilot Library, we found two hit candidates of Fv/Fm inhibitor which showed considerably high inhibitory activity in dose dependent manner. Compounds having structurally related compounds (analogues) or partial structures of these compounds were found to exhibit the same photosynthetic inhibitory activity as the compounds obtained by the screening. Moreover, the photosynthetic parameters by chlorophyll fluorescence revealed that one compound inhibits the PSII itself and another compound inhibits somewhere downstream of the PSII.
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