| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Cell-to-cell direct cloning technology has established by designed horizontal transfer using extra-cellular DNA without purified DNAs. The purified DNAs in a test tube have been generally required to introduce into the host cell for molecular cloning technology in laboratory. In the natural environment, extra-cellular DNAs without purified step contribute to the gene delivery during horizontal gene transfer (HGT) dependent on the stability of DNA released from donor cells. Under such a concept we have developed that DNA delivery procedure using lysate of Escherichia coli lysed by virulent bacteriophage into competent Bacillus subtilis cells. The direct cloning technology has been applied for the DNA synthesis by fragment assembly within the B. subtilis genome. Furthermore we showed the transfer of plasmid DNAs from lysing E. coli into cyanobacteria, which is suggests the procedure is useful for general use.
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