Identification and collection of actinomycete promoter sequences by TSS-seq

• Project/Area Number: 26430201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: System genome science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Ishikawa Jun 国立感染症研究所, 真菌部, 室長 (40202957)
• Co-Investigator(Kenkyū-buntansha): 星野 泰隆 国立感染症研究所, 真菌部, 主任研究官 (40399457)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 転写開始点 / ゲノム工学 / 抗生物質 / ゲノム

Outline of Final Research Achievements

Recently, discovery and development of novel antibiotics from actinomycetes is performed by genomic approaches. However there are still not enough technology for engineering antibiotic-producing actinomycetes, especially promoter for gene expression. In this work, by using NGS, we identified many transcriptional start sites (TSSs) in a Streptomyces genome and estimated putative consensus sequences of streptomyces promoter. From 18-hr culture, E. coli's Pribnow-like hexamers were found at surrounding -14 position. These findings may contribute to genomic engineering in actinomycetes,including the development of novel antibiotics.

Research Products

• [Remarks] Streptomyces Genome Project Server
  • URL: http://streptomyces.nih.go.jp/