Sperm banking of wild animals using freeze-drying technique

• Project/Area Number: 26430204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservation of biological resources
• Research Institution: Kyoto University
• Principal Investigator: Kaneko Takehito 京都大学, 医学研究科, 特定講師 (30332878)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: フリーズドライ / 凍結乾燥 / 配偶子保存

Outline of Final Research Achievements

Freeze-dried sperm can be stored for long term at 4 degree C. The aim of this study is establishment of sperm banking for wild animals using freeze-drying technique. In this study, sperm that collected from various wild animals were freeze-dried, and their fertility was examined. Sperm were freeze-dried using Tris-EDTA solution. Freeze-dried sperm were stored at 4 degree C. In this study, all sperm were maintained their fertility. These results showed that the sperm freeze-drying could be applied for preserving sperm of wild animals.

Research Products

• [Journal Article] Sperm freeze-drying and micro-insemination for biobanking and maintenance of genetic diversity in mammals (2016)
  • Author(s): Takehito Kaneko
  • Journal Title: Reproduction Fertility and Development Volume: 28 Issue: 8 Pages: 1079-1087
  • DOI: 10.1071/rd15386
  • Peer Reviewed
• [Journal Article] In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability (2016)
  • Author(s): Hiroaki Taketsuru, Takehito Kaneko
  • Journal Title: Journal of Reproduction and Develoment Volume: 62 Pages: 521-526
  • NAID: 130005431276
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simple gamete preservation and artificial reproduction of mammals using micro-insemination techniques (2015)
  • Author(s): Takehito Kaneko
  • Journal Title: Reproductive Medicine and Biology Volume: 14 Pages: 99-105
  • Peer Reviewed
• [Journal Article] Sperm preservation by freeze-drying for the conservation of wild animals (2014)
  • Author(s): Kaneko T, Ito H, Sakamoto H, Onuma m, Inoue-Murayama M
  • Journal Title: PLOS ONE Volume: 9 Issue: 11 Pages: e113381-e113381
  • DOI: 10.1371/journal.pone.0113381
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] フリーズドライによる哺乳動物精子長期保存法の開発とその応用 (2016)
  • Author(s): 金子武人
  • Organizer: Cryopreservation Conference 2016
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2016-11-10
  • Invited
• [Presentation] Dry-state preservation of mammalian sperm (2016)
  • Author(s): Takehito Kaneko
  • Organizer: Cryobiology course: Cryopreservation of gametes, embryos and stem cells
  • Place of Presentation: ベルギー
  • Year and Date: 2016-09-20
  • Int'l Joint Research / Invited
• [Presentation] ラットにおける生殖工学技術 (2016)
  • Author(s): 金子武人
  • Organizer: 千里ライフサイエンス技術講習会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2016-09-01
  • Invited
• [Presentation] Latest reproductive technologies in mammals (2016)
  • Author(s): Takehito Kaneko
  • Organizer: From Zygote to Blastocyst: Immunosurgery, Stem Cell Derivation and Transgenics
  • Place of Presentation: イギリス
  • Year and Date: 2016-08-18
  • Int'l Joint Research / Invited
• [Presentation] Dry-state preservation of mammalian sperm (2016)
  • Author(s): Takehito Kaneko
  • Organizer: 42nd Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: アメリカ・ケンタッキー州
  • Year and Date: 2016-01-23
  • Int'l Joint Research / Invited
• [Presentation] ニワトリフリーズドライ精子の活用法の検討 (2015)
  • Author(s): 鏡味裕、胡桃澤希未、西本優里、仲谷隆馬、金子武人
  • Organizer: 日本畜産学会第120会大会
  • Place of Presentation: 北海道
  • Year and Date: 2015-09-11
• [Presentation] ネコの未成熟精子およびフリーズドライ精子による顕微授精 (2015)
  • Author(s): 辻本恭典、鳩谷晋吾、金子武人、杉浦喜久弥、稲葉俊夫
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 青森県
  • Year and Date: 2015-09-07
• [Presentation] ウシ科動物から採取した精子のフリーズドライおよび凍結保存法の開発 (2015)
  • Author(s): 金子武人、田中優、米澤彩、浅川卓也 、鈴木勲
  • Organizer: 第21回日本野生動物医医学会大会
  • Place of Presentation: 北海道
  • Year and Date: 2015-07-31
• [Presentation] Sperm preservation by freeze-drying in endangered animals (2015)
  • Author(s): Takehito Kaneko
  • Organizer: 41th Annual conference of the international embryo transfer society
  • Place of Presentation: Versailles, France
  • Year and Date: 2015-01-10 – 2015-01-13
• [Presentation] Sperm banking using freeze-drying in endangered animals (2014)
  • Author(s): Takehito Kaneko, Hideyuki Ito, Hidefusa Sakamoto, Manabu Onuma, Miho Murayama
  • Organizer: Unraveling biodiversity from DNA
  • Place of Presentation: Ibaraki, Japan
  • Year and Date: 2014-09-19
• [Presentation] 希少動物におけるフリーズドライ精子保存法の確立及び配偶子バンクの設立 (2014)
  • Author(s): 金子武人、伊藤英之、坂本英房、大沼学、村山美穂
  • Organizer: 第20回日本野生動物医学会大会
  • Place of Presentation: つくば市
  • Year and Date: 2014-09-16 – 2014-09-19
• [Presentation] Preservation of mammalian sperm by freeze-drying (2014)
  • Author(s): Takehito Kaneko
  • Organizer: 2014 World forum on biology
  • Place of Presentation: Savannah, GA, USA
  • Year and Date: 2014-05-31 – 2014-06-04
  • Invited
• [Book] Method Molecular biology, Chromosomal Mutagenesis (2015)
  • Author(s): Takehito Kaneko
  • Total Pages: 333
  • Publisher: Humana Press
• [Remarks] ラット・マウスの生殖学/生殖工学研究のページ
  • URL: http://www.anim.med.kyoto-u.ac.jp/reproduction/home_jp.aspx
• [Remarks] フリーズドライ（凍結乾燥）精子で希少野生動物保護 －京都発・希少野生動物配偶子バンク－
  • URL: http://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013/130827_1.htm