| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we examined for the purpose of development of novel technique on vacuum drying-room temperature preservation in mouse preimplantation stage embryos. Mouse embryos were vacuum-dried with stock solution containing various concentration of trehalose and preserved at room temperature for 48 h. After rehydration, the efficiency of survived embryos were very low when none of embryos vacuum-dried and preserved at room temperature for 72 h were survived. In addition, knockin (KI) mice inserted trehalose transporter gene into safe harbor region of ROSA26 gene locus were generated by CRISPR/Cas9 system. Preimplantation stage embryos from trehalose transporter-KI mice will be vacuum dried and preserved at room temperature for confirming their survivability after rehydration.
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