| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Histone chaperones cooperatively function for chromatin structure regulation. In this project, we have clarified the mechanism of chromatin regulation by FACT complex. From proteomics analysis,Pdr1, HP1, and Fip1 were identified as FACT associating factors, and chromatin structural changes caused by conjugation with FACT were analyzed. As Pdr1 is a transcription factor that activates the xenobiotics elimination system, it became clear that this activator for the efflux pump system transiently evicts the nucleosome from the promoter chromatin, and reorganizes chromatin structure via FACT.
HP1 (Heterochromatin Protein 1) is known as a platform of heterochromatin forming factors. Our analysis revealed that it stabilizes the nucleosome octamer through the physical interaction with FACT complex at histone H3K9 methylated loci.
Fip1 analysis is currently underway. Our data related to chromatin silencing was obtained near the boundary of CnpA / H3 at the pericentromeric heterochromatin.
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