| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Following three points should be stressed as novel findings. As to the effects of MCM4 mutations on MCM2-7 complex formation, mutations from cancer cells affect MCM complex formation through changes in interaction with other MCM members. Furthermore, it is suggested that the presence of the mutant MCM4 affects DNA replication in HeLa cells. It has been shown that a number of MCM-interacting proteins bind to conserved MCM-box, suggesting that these proteins regulate MCM function through the activity. The amino-terminal region of MCM4 is shown to be required for DNA helicase activity of MCM4/6/7 complex. It is suggested that the phosphorylation of the region with CDK can destabilize MCM complex.
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