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Study on chromosome architecture and regulation in germ cell

Research Project

Project/Area Number 26440003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionKumamoto University (2016)
Keio University (2014-2015)

Principal Investigator

Kei-ichiro Ishiguro  熊本大学, 発生医学研究所, 准教授 (30508114)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生殖細胞 / 減数分裂 / 胚性幹細胞 / 着床前胚 / 染色体 / 着床前前胚 / ES細胞 / oocyte / 着床前初期胚 / ES 細胞
Outline of Final Research Achievements

ZSCAN4 is expressed specifically in preimplantation embryos in vivo and ES cells in vitro. However, expression patterns of mouse ZSCAN4 in vivo have been largely elusive. We showed that ZSCAN4 proteins are expressed not only in preimplantation embryo but also in adult ovaries and testes. In ovaries, ZSCAN4 proteins were detected in germinal vesicle stage oocytes, indicating that Zscan4 loci are activated during the late stage in meiotic prophase I. Remarkably, ZSCAN4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization. These spatiotemporal difference in ZSCAN4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocytes maturation. In testes, ZSCAN4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that ZSCAN4 may play critical roles during late meiotic prophase in germ cells.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (2 results) Book (2 results) Remarks (2 results)

  • [Int'l Joint Research] Fondazione IRCCS(Italy)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Research Center for/Regenerative Medicine(Italy)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos2017

    • Author(s)
      Ishiguro K., Nakatake Y., Chikazawa-Nohtomi N., Kimura H., Akiyama T., Oda M., Ko SBH., Ko MSH.
    • Journal Title

      In Vitro Cell.Dev.Biol.-Anim.

      Volume: 53 Issue: 2 Pages: 179-190

    • DOI

      10.1007/s11626-016-0097-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis2017

    • Author(s)
      Ishiguro K., Monti M., Akiyama T., Kimura H., Chikazawa-Nohtomi N., Sakota M., Sato S., Redi CA., Ko SBH., Ko MSH.
    • Journal Title

      In Vitro Cell.Dev.Biol.-Anim.

      Volume: 53 Issue: 2 Pages: 167-178

    • DOI

      10.1007/s11626-016-0096-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 減数第一分裂に特異的な染色体分配を制御する新規動原体因子MEIKIN2015

    • Author(s)
      石黒啓一郎, 金智慧, 渡邊嘉典
    • Journal Title

      実験医学

      Volume: 33 Pages: 1427-1431

    • Related Report
      2015 Research-status Report
  • [Journal Article] マウスES細胞の染色体安定性を制御するエピゲノム機構2015

    • Author(s)
      秋山智彦, 小田真由美, 石黒啓一郎, 洪繁, 洪実
    • Journal Title

      細胞工学

      Volume: 34 Pages: 1052-1056

    • Related Report
      2015 Research-status Report
  • [Journal Article] MEI4: a central player in the regulation of meiotic DNA double strand break formation in the mouse2015

    • Author(s)
      Kumar R., Ghyselinck N., Ishiguro K., Watanabe Y., Kouznetsova A., Höög C., Strong E., Schimenti J., Daniel K., Toth A., de Massy B.
    • Journal Title

      Journal of Cell Science

      Volume: 128 Pages: 1800-1811

    • DOI

      10.1242/jcs.165464

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Meikin is a conserved regulator of meiosis-I-specific kinetochore function2015

    • Author(s)
      Kim, J., Ishiguro, K., Nambu, A., Akiyoshi, B., Yokobayashi, S., Kagami, A., Ishiguro, T., Pendas, A.M., Takeda, N., Sakakibara, Y., Kitajima, T.S., Tanno, Y., Sakuno, T., and Watanabe, Y.
    • Journal Title

      Nature

      Volume: 517 Issue: 7535 Pages: 466-471

    • DOI

      10.1038/nature14097

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex2014

    • Author(s)
      Belzil C., Asada N., Ishiguro K., Nakaya T., Parsons K., Pendolino V., Neumayer G., Mapelli M., Nakatani Y., Sanada K., Nguyen MD.
    • Journal Title

      Biology OPEN

      Volume: 3 Issue: 6 Pages: 475-485

    • DOI

      10.1242/bio.20147807

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ZSCAN4 is expressed not only in preimplantation embryos but also in adult ovaries and testes2016

    • Author(s)
      Kei-ichiro Ishiguro, Manuela Monti, Tomohiko Akiyama, Hiromi Kimura, Nana Chikazawa-Nohtomi, Miki Sakota, Saeko Sato, Carlo Alberto Redi, Shigeru B.H. Ko, Minoru S.H. Ko
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Meikin is a conserved regulator of meiosis-I-specific kinetochore function2014

    • Author(s)
      Kim J, Ishiguro K., Nambu A., Sakakibara Y., Kitajima T.S., Watanabe Y.
    • Organizer
      第32回染色体ワークショップ
    • Place of Presentation
      広島廿日市市 安芸グランドホテル
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Research-status Report
  • [Book] 実験医学2015

    • Author(s)
      石黒啓一郎, 金智慧、渡邊嘉典
    • Total Pages
      5
    • Publisher
      羊土社
    • Related Report
      2014 Research-status Report
  • [Book] ライフサイエンス新着論文レビュー2015

    • Author(s)
      石黒啓一郎, 金智慧、渡邊嘉典
    • Total Pages
      6
    • Publisher
      大学共同利用機関法人 情報・システム研究機構ライフサイエンス統合データベースセンター
    • Related Report
      2014 Research-status Report
  • [Remarks] 熊本大学発生医学研究所 染色体制御分野 分野紹介

    • URL

      http://www.imeg.kumamoto-u.ac.jp/bunya_top/chromosome-biology/seika/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 慶應義塾大学医学部 システム医学講座

    • URL

      http://systemsmedicine.jp/about/

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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