Genome-wide screening of the non-flagellar genes involved in regulation of the flagellar biosynthesis and function in Escherichia coli and Salmonella
| Project/Area Number |
26440006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Research Collaborator |
MIYAGAWA Akira 岡山大学, 大学院自然科学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 分子遺伝学 / 遺伝子発現 / 鞭毛レギュロン / グローバル制御 / 制御ネットワーク / 鞭毛回転 / 網羅的検索 / c-di-GMP / 遺伝子発現制御 / 鞭毛回転制御 |
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| Outline of Final Research Achievements |
This study was carried out to identify all the E. coli non-flagellar genes which are potentially involved in regulation of flagellar biosynthesis and function. It was found that nearly 10% of the genes have negative or positive effects on flagellar gene expression. Most of them were shown to regulate the expression of the flagellar genes via transcriptional control of the class 1 flagellar operon. Newly identified six genes were shown to encode negative regulators specific for transcription of the class 2 flagellar operons. Interestingly, some genes were found to exert negative effects on flagellar function without significant effects on flagellar gene expression.
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Report
(4 results)
Research Products
(2 results)
