Genome-wide screening of the non-flagellar genes involved in regulation of the flagellar biosynthesis and function in Escherichia coli and Salmonella

• Project/Area Number: 26440006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Okayama University
• Principal Investigator: KUTSUKAKE Kazuhiro 岡山大学, 自然科学研究科, 教授 (90143362)
• Research Collaborator: MIYAGAWA Akira 岡山大学, 大学院自然科学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 分子遺伝学 / 遺伝子発現 / 鞭毛レギュロン / グローバル制御 / 制御ネットワーク / 鞭毛回転 / 網羅的検索 / c-di-GMP / 遺伝子発現制御 / 鞭毛回転制御

Outline of Final Research Achievements

This study was carried out to identify all the E. coli non-flagellar genes which are potentially involved in regulation of flagellar biosynthesis and function. It was found that nearly 10% of the genes have negative or positive effects on flagellar gene expression. Most of them were shown to regulate the expression of the flagellar genes via transcriptional control of the class 1 flagellar operon. Newly identified six genes were shown to encode negative regulators specific for transcription of the class 2 flagellar operons. Interestingly, some genes were found to exert negative effects on flagellar function without significant effects on flagellar gene expression.

Research Products

• [Presentation] 鞭毛レギュロンを制御するグローバルネットワーク (2015)
  • Author(s): 宮川暁，沓掛和弘
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 大腸菌鞭毛レギュロンのSdiA蛋白質による発現制御 (2014)
  • Author(s): 幡基友紀，沓掛和弘
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 長浜バイオ大学（滋賀県長浜市）
  • Year and Date: 2014-09-17 – 2014-09-19