| Project/Area Number |
26440007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISHITOU HIDEKI 宮崎大学, 医学部, 教授 (00332627)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヒストン / クロマチン / ヘテロクロマチン / DNA修復 / クロマチン動態 |
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| Outline of Final Research Achievements |
RbAp46/48 family, histone chaperone, is evolutionarily conserved WD40 proteins, which are involved in various chromatin-metabolizing process, but their in vivo functional relevance is yet unclear. To examine the biological role of pRbAp48, we generated a conditional RbAp48-knokout cells. Depletion of RbAp48 led to delayed S phase progression associated with slow DNA synthesis and nascent nucleosome formation, followed by cell death. Prior to cell death, these cells exhibited aberrant mitosis such as highly condensed and abnormal chromosome alignment on the metaphase plate, leading to chromosome missegregation, concomitant with loss of HP1 association to pericentromere and elevated levels of acetylation and sligtly decreased levels of methylation, specifically at Lys-9 residue of histone H3. These results suggest that RbAp48 plays an important role for the chromosome stability in proper organization of heterochromatin structure through the regulation of epigenetic mark.
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