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Reconstitution of mitotic chromosomes with purified factors

Research Project

Project/Area Number 26440012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Shintomi Keishi  国立研究開発法人理化学研究所, 平野染色体ダイナミクス研究室, 研究員 (60462694)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords染色体 / ヒストン / ヌクレオソーム / コンデンシン / 再構成 / カエル卵無細胞系 / カエル卵抽出液 / ヒストンシャペロン / 分裂期 / トポⅡ / 細胞周期
Outline of Final Research Achievements

The nucleosome is the fundamental structural units of eukaryotic chromatin. Immediately before cell division, duplicated nucleosome fibers are organized into a pair of rod-shaped structures within a mitotic chromosome. However, it remains unclear whether nucleosome assembly is indeed an essential prerequisite for mitotic chromosome assembly. To settle this naive question, I have established chromosome reconstitution system with a minimum set of purified proteins and the cell-free chromosome assembly assay made from frog eggs. A substantial set of our results convincingly indicates that, although formation of chromosome axes does not rely on nucleosomes, functional interaction between nucleosomes and the multi-protein complexes termed condensins is required for assembly of fully compact chromosomes.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014 Other

All Journal Article (2 results) (of which Acknowledgement Compliant: 2 results,  Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] A sister chromatid cohesion assay using Xenopus egg extracts2017

    • Author(s)
      Keishi Shintomi, Tatsuya Hirano
    • Journal Title

      Methods in Molecular Biology

      Volume: 1515 Pages: 3-21

    • DOI

      10.1007/978-1-4939-6545-8_1

    • ISBN
      9781493965434, 9781493965458
    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Reconstitution of mitotic chromatids with a minimum set of purified factors2015

    • Author(s)
      Keishi Shintomi, Tatsuro S. Takahashi, and Tatsuya Hirano
    • Journal Title

      Nature Cell Biology

      Volume: 17 Issue: 8 Pages: 1014-1023

    • DOI

      10.1038/ncb3187

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 分裂期染色体構築におけるヌクレオソームの役割2017

    • Author(s)
      新冨圭史、井上玄志、大隅圭太、大杉美穂、平野達也
    • Organizer
      第34回染色体ワークショップ・第15回核ダイナミクス研究会
    • Place of Presentation
      かずさアカデミアホール(千葉県木更津市)
    • Year and Date
      2017-01-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒストンはどのように染色体構築に貢献しているのか?2016

    • Author(s)
      新冨圭史、井上玄志、大杉美穂、大隅圭太、平野達也
    • Organizer
      第33回染色体ワークショップ・第14回核ダイナミクス研究会
    • Place of Presentation
      松島一の坊(宮城県松島町)
    • Year and Date
      2016-01-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Large-scale assembly of mitotic chromatids depends on nucleosome dynamics2015

    • Author(s)
      Keishi Shintomi and Tatsuya Hirano
    • Organizer
      International Symposium on Chromatin Structure, Dynamics, and Function
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of mitotic chromatids with a minimum set of purified factors2015

    • Author(s)
      Keishi Shintomi, Tatsuro S. Takahashi, and Tatsuya Hirano
    • Organizer
      EMBO workshop, SMC proteins
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2015-05-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reconstitution of mitotic chromatids with a minimum set of purified factors2015

    • Author(s)
      Keishi Shintomi
    • Organizer
      EMBO workshop - SMC proteins
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2015-05-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Reconstitution of mitotic chromatids with a minimum set of purified components2014

    • Author(s)
      Keishi Shintomi
    • Organizer
      The 9th 3R symposium
    • Place of Presentation
      Gotemba, Japan
    • Year and Date
      2014-11-19
    • Related Report
      2014 Research-status Report
  • [Remarks] 研究室ウェブサイト

    • URL

      http://www.riken.jp/chromdyna/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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