Reconstitution of mitotic chromosomes with purified factors
| Project/Area Number |
26440012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shintomi Keishi 国立研究開発法人理化学研究所, 平野染色体ダイナミクス研究室, 研究員 (60462694)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 染色体 / ヒストン / ヌクレオソーム / コンデンシン / 再構成 / カエル卵無細胞系 / カエル卵抽出液 / ヒストンシャペロン / 分裂期 / トポⅡ / 細胞周期 |
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| Outline of Final Research Achievements |
The nucleosome is the fundamental structural units of eukaryotic chromatin. Immediately before cell division, duplicated nucleosome fibers are organized into a pair of rod-shaped structures within a mitotic chromosome. However, it remains unclear whether nucleosome assembly is indeed an essential prerequisite for mitotic chromosome assembly. To settle this naive question, I have established chromosome reconstitution system with a minimum set of purified proteins and the cell-free chromosome assembly assay made from frog eggs. A substantial set of our results convincingly indicates that, although formation of chromosome axes does not rely on nucleosomes, functional interaction between nucleosomes and the multi-protein complexes termed condensins is required for assembly of fully compact chromosomes.
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Report
(4 results)
Research Products
(9 results)
