| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Ca2+ pump transports Ca2+ at transport sites of membrane domain coupled to ATP hydrolysis at catalytic site of cytoplasmic domain. The structural roles of M2 helix connecting these domains were investigated by use of site-directed mutagenesis. The results indicated that cytoplasmic region (M2c) and membrane region (M2m) of M2 and junctional loop to cytoplasmic domain have specific functions at specific reaction steps, which enable transmission of structural changes between these domains. The functional roles of Gly105 at connecting region of M2c-M2m were investigated by site-directed substitutions. The results indicated that in the phosphorylated-intermediate isomerization Gly105 with its flexibility absorbs distortion of M2 caused by large structural changes at cytoplasmic domain, and that this enables proper Ca2+-gating and coupled transport.
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