Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Outline of Final Research Achievements |
The kinetic and structural analyses of β-1,4-mannosyl-N-acetyl-glucosamine phosphorylase which is a key enzyme in novel metabolic pathway for N-glycan where phosphorylase participates, were carried out to elucidate this metabolic pathway in intestinal bacteria. The enzymes from some microbes have strict regioselectivity and acceptor specificity against N-acetyl-glucasamine and N,N’-diacetyl-chitobiose as acceptors in reverse phosphorolysis. The three-dimensional structure of β-1,4-mannosyl-N-acetyl-glucosamine phosphorylase from Bacteroides thetaiotaomicron has not yet been determined. This structure analysis is carried out continuously now. To investigate of an uncharacterized gene which is predicted to participate in N-glycan metabolism existing close to phosphorylase gene, the hydrolytic reaction of the protein encoded by this gene was examined using various N-glycans as the substrate candidates. However, this protein did not show hydrolytic reaction.
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