Studies on molecular mechanism of paracellular pathway
| Project/Area Number |
26440029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KONDOH Masuo 大阪大学, 大学院薬学研究科, 准教授 (50309697)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Ｘ線結晶構造解析 / 膜タンパク質 / 国際情報交換 |
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| Outline of Final Research Achievements |
We performed the following studies on claudin, which plays a barrier function between cells, and connexin, which is responsible for substrate transport between cells. Two CL binders wirh high affinity for CL were created from mutant variant screening of the C-terminal domain of Clostridium perfringens enterotoxin, which is known as a inhibitory protein in the paracellular route. These X-ray structures revealed strucrutal changes contributing to the stability of complex formation with CL. For structural activity correlation was analyzed for tisse-specifically expressed Cx by the combination of dual patch clamp and homology structure modelling.
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Report
(4 results)
Research Products
(10 results)
