Analysis of molecular association and dissociation mechanism in replication restart
| Project/Area Number |
26440031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Abe Yoshito 九州大学, 薬学研究院, 准教授 (60315091)
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| Co-Investigator(Kenkyū-buntansha) |
片山 勉 九州大学, 薬学研究院, 教授 (70264059)
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| Research Collaborator |
UEDA Tadashi 九州大学, 大学院薬学研究院, 教授
SHIROISHI Mitsunori 九州大学, 大学院薬学研究院, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 複製再開始プライモソーム / タンパク質-DNA複合体 / タンパク質構造 / PriC / PriB / DnaT / DnaB / 蛋白質構造 / 蛋白質-DNA複合体 |
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| Outline of Final Research Achievements |
In DNA replication restart, systematic association and dissociation of several proteins are occurred on DNA. In this application, we performed the structure and function analysis of the proteins (PriA、PriB、DnaT、PriC、DnaB ) involved in replication restart in Escherichia coli. In DnaT, we examined the oligomeric state of the N-terminal domain and the ssDNA (singles-stranded DNA) dissociation activity from PriB-ssDNA (singles-stranded DNA) complex by the linker region between the N-terminal and C-terminal domains, which is involved in the binding to PriB. Moreover, we examined the interaction between PriC and DnaB. Together with these results, we contributed elucidating the mechanism in replication restart.
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Report
(4 results)
Research Products
(21 results)
