Regulation of peroxisome biogenesis by protein phosphorylation and signal transduction
Project/Area Number |
26440032
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Kyushu University |
Principal Investigator |
Okumoto Kanji 九州大学, 理学研究院, 助教 (20363319)
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Co-Investigator(Renkei-kenkyūsha) |
FUJIKI Yukio 九州大学, 生体防御医学研究所, 特任教授 (70261237)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | ペルオキシソーム / リン酸化 / タンパク質輸送 / 一次繊毛 / 繊毛病 / タンパク質 / シグナル伝達経路 |
Outline of Final Research Achievements |
A peroxisomal membrane protein Pex14p plays a pivotal role in peroxisomal matrix protein import. In this study, we found that Pex14p is phosphorylated upon oxidative stresses. Phosphorylation of Pex14p regulates import of peroxisomal matrix proteins, which can counteract cellular oxidative stresses. We also found that NDR2, a protein kinase involved in primary cilium formation, partially localized to peroxisomes in a manner dependent on its peroxisome-targeting signal-1-like sequence, Gly-Lys-Leu, at the C-terminus. Novel peroxisomal function is implicated in ciliogenesis and pathogenesis of ciliopathies.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] The VDAC2-BAK axis regulates peroxisomal membrane permeability.2017
Author(s)
Hosoi, K., Miyata, N., Mukai, S., Furuki, S., Okumoto, K., Cheng, E. H., and *Fujiki, Y.
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Journal Title
J. Cell Biol.
Volume: 216
Issue: 3
Pages: 709-722
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Defining dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae.2017
Author(s)
Imoto, Y., Abe, Y., Okumoto, K., Honsho, M., Kuroiwa, H., Kuroiwa, T., and *Fujiki, Y.
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Journal Title
J. Cell Sci.
Volume: 130
Pages: 853-867
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Localization of protein kinase NDR2 to peroxisomes and its role in ciliogenesis.2017
Author(s)
Abe, S., Nagai, T., Masukawa, M., Okumoto, K., Homma, Y., Fujiki, Y., and Mizuno, K.
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Journal Title
J. Biol. Chem.
Volume: 292
Issue: 10
Pages: 4089-4098
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system2017
Author(s)
Okumoto, K., Honsho, M., Liu, Y., and Fujiki, Y.
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Journal Title
In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.), Springer, Humana Press, New York, USA
Volume: 1595
Pages: 213-219
DOI
ISBN
9781493969357, 9781493969371
Related Report
Peer Reviewed
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[Journal Article] Blue-Native PAGE: Applications to study on peroxisome biogenesis2017
Author(s)
Okumoto , K., Tamura, S., and Fujiki, Y.
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Journal Title
In: Schrader, M. (ed.) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed.: Walker, J.M.), Springer, Humana Press, New York, USA
Volume: 1595
Pages: 197-205
DOI
ISBN
9781493969357, 9781493969371
Related Report
Peer Reviewed
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[Journal Article] Mild reduction of plasmalogens causes rhizomelic chondrodysplasia punctata: Functional characterization of a novel mutation2014
Author(s)
Noguchi, M., Honsho, M., Abe, Y., Toyama, R., Niwa, H., Sato, Y., Ghaedi, K., Rahmanifar, A., Shafeghati, Y., and *Fujiki, Y.
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Journal Title
Journal of Human Genetics
Volume: 59
Issue: 7
Pages: 387-392
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular basis for peroxisome biogenesis disorders2014
Author(s)
*Fujiki, Y., Okumoto, K., Mukai, S., and Tamura, S.
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Journal Title
In: Brocard, C. and Hartig, A. (eds) Molecular machines involved in peroxisome biogenesis and maintenance, Springer-Verlag, Wien, Austria
Volume: ―
Pages: 91-110
DOI
ISBN
9783709117873, 9783709117880
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Biogenesis of peroxisome: assembly of nascent membrane proteins2016
Author(s)
Liu, Y., Yagita, Y., Okumoto, K., Shinohara, K., Nagata, A., and Fujiki, Y.
Organizer
2016 ASCB Annual Meeting
Place of Presentation
San Fransisco, USA
Year and Date
2016-12-03
Related Report
Int'l Joint Research
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[Presentation] Peroxisome biogenesis: Import of nascent membrane and matrix proteins2016
Author(s)
Liu, Y., Tamura, S., Okumoto, K., Yagita, Y., Kawamura, Y., Nagata, A., and Fujiki, Y.
Organizer
第39回日本分子生物学会
Place of Presentation
パシフィコ横浜(神奈川)
Year and Date
2016-11-30
Related Report
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[Presentation] Ultrastructure and molecular mechanism of Ring-shaped Supramolecular Nanomachinery for Peroxisome Division Revealed by Single Organelle EM Analysis2016
Author(s)
Imoto, Y., Abe, Y., Yoshida, M., Honsho, M., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., and Fujiki, Y.
Organizer
1st Cyanidioschyzon merolae Symposium
Place of Presentation
東京大学(東京)
Related Report
Int'l Joint Research
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