| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Copper amine oxidases (CAOs) catalyze the oxidation of various aliphatic amines to the corresponding aldehydes, ammonia and hydrogen peroxide. We focused on the structural insights into the catalytic mechanism of a CAO from Arthrobacter globiformis (AGAO) using an ultra-high resolution X-ray and neutron crystallography. We determined the structure of oxidized-form (substrate-free form) of AGAO at 1.72/1.14 angstrom by neutron/X-ray crystallography. Based on the precise three-dimensional structure information obtained, we got new knowledge on reaction mechanism accompanied by structural change and fluctuation of AGAO. Further, we also obtained the neutron and X-ray diffraction data of semiquinone intermediate. We are currently refining the structure.
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