| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Granule-bound starch synthase I(GBSSI) catalyze the amylose (α-1,4-linked glucose chain ) elongation process by adding glucose from nucleotide diphosphate glucose. We conducted the structural analysis of rice GBSSI in complex with maltooligosaccharides having the α-1,4-linked glucose moieties to clarify the substrate-recognition mechanism of the plant enzyme. The structures had two Rossmann fold N- and C-domains, connected by the canonical two hinge peptides, and an interdomain disulfide bond that appears to be conserved in the Poaceae plant family. The catalytic site appeared to be located in the concave of two domains, and the flexible loop regions near the catalytic concave appeared to be the carbohydrate-binding site.
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