| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Microbial rhodopsins are photoactive membrane proteins containing the chromophore retinal. Upon light absorption, they undergo the cyclic photoreactions and exert respective functions such as various ion transports and the light signal transductions. Here, we performed functional analyses of the outward Na+ pumping rhodopin (NaR). The results are summarized as follows: 1) There exists a cation binding site on the NaR surface. The cation binding distorts the protein structure in the dark state, and then influences the early intermediates formed after photoexcitation. 2) Na+ uptake and release reactions were directly detected by the photo-induced changes of Na+ concentration. These reactions occurred almost simultaneously with the formation and decay of O intermediate, respectively.
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