Study on the physiological significance and the molecular mechanism of Na+-pumping rhodopsin
Project/Area Number |
26440042
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
|
Research Institution | Hokkaido University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
宮内 正二 東邦大学, 薬学部, 教授 (30202352)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | ロドプシン / ナトリウムポンプ / フォトサイクル / レチナール / 膜蛋白質 / イオンポンプ / 微生物ロドプシン |
Outline of Final Research Achievements |
Microbial rhodopsins are photoactive membrane proteins containing the chromophore retinal. Upon light absorption, they undergo the cyclic photoreactions and exert respective functions such as various ion transports and the light signal transductions. Here, we performed functional analyses of the outward Na+ pumping rhodopin (NaR). The results are summarized as follows: 1) There exists a cation binding site on the NaR surface. The cation binding distorts the protein structure in the dark state, and then influences the early intermediates formed after photoexcitation. 2) Na+ uptake and release reactions were directly detected by the photo-induced changes of Na+ concentration. These reactions occurred almost simultaneously with the formation and decay of O intermediate, respectively.
|
Report
(4 results)
Research Products
(19 results)
-
[Journal Article] Photochemical characterization of actinorhodopsin and its functional existence in the natural host2016
Author(s)
Nakamura, S., Kikukawa, T., Tamogami, J., Kamiya, M., Aizawa, T., Hahn, M., Ihara, K., Kamo, N., Demura, M.
-
Journal Title
BBA-BIOENERGETICS
Volume: 1857
Issue: 12
Pages: 1900-1908
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Probing the Cl--pumping photocycle of pharaonis halorhodopsin: Examinations with bacterioruberin, an intrinsic dye, and membrane potential-induced modulation of the photocycle2015
Author(s)
Kikukawa, T., Kusakabe, C., Kokubo, A., Tsukamoto, T., Kamiya, M., Aizawa, T., Ihara, K., Kamo, N., Demura, M.
-
Journal Title
Biochim. Biophys. Acta
Volume: 1847
Issue: 8
Pages: 748-758
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] The effects of chloride ion binding on the photochemical properties of sensory rhodopsin II from Natronomonas pharaonis2014
Author(s)
Tamogami, J., Iwano, K., Matsuyama, A., Kikukawa, T., Demura, M., Nara, T., Kamo, N.
-
Journal Title
J. Photochem. Photobiol. Sci.
Volume: 141
Pages: 192-201
DOI
Related Report
Peer Reviewed
-
[Journal Article] Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide2014
Author(s)
Takahiro Kushibiki, Masakatsu Kamiya, Tomoyasu Aizawa, Yasuhiro Kumaki, Takashi Kikukawa, Mineyuki Mizuguchi, Makoto Demura, Shun-ichiro Kawabata, Keiichi Kawano
-
Journal Title
Biochimica et Biophysica Acta - Proteins and Proteomics
Volume: 1,844
Issue: 3
Pages: 527-534
DOI
Related Report
Peer Reviewed
-
[Presentation] Solid-state NMR analysis of sodium ion pump rhodopsin and its Na+-binding2016
Author(s)
Tamaki, H., Saito, Y., Egawa, A., Kikukawa, T., Fujiwara, T., Demura, M.
Organizer
17th International Conference on Retinal Proteins
Place of Presentation
ポツダム(ドイツ)
Year and Date
2016-10-03
Related Report
Int'l Joint Research
-
[Presentation] Replacements of “donor” residues in the light- driven H+-pump rhodopsins2016
Author(s)
Nishiya, K., Sasaki, S., Tamogami, J., Kikukawa, T., Aizawa, T., Kamo, N., Demura, M.
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県・筑波)
Related Report
-
-
-
[Presentation] Inversion of sequence and direction of the proton transfer in proteorhodopsin at high pH2015
Author(s)
Tamogami, J., Kikukawa, T., Nara, T., Demura, M., Muneyuki, E., Kamo, N.
Organizer
The 2015 International Chemical Congress of Pacific Basin Societies
Place of Presentation
Honolulu (USA)
Year and Date
2015-12-19
Related Report
Int'l Joint Research
-
[Presentation] Conversion of a novel chloride pumping rhodopsin to a proton pump with a single amino acid replacement2015
Author(s)
Hasemi, T., Kikukawa, T., Kamiya, M., Aizawa, T., Kamo, N., Demura, M.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県・金沢)
Year and Date
2015-09-13
Related Report
-
[Presentation] Functionally important residues in the cytoplasmic half channel of light-driven Cl- pump Natronomonas pharaonis halorhodopsin2015
Author(s)
Shimosaka, A., Kikukawa, T., Kamiya, M., Aizawa, T., Kamo, N., Demura, M.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県・金沢)
Year and Date
2015-09-13
Related Report
-
[Presentation] Photochemistry of the H+-pumping rhodopsin from freshwater Actinobacteria2014
Author(s)
Nakamura, S., Kikukawa, T., Kamiya, M., Aizawa, T., Hahn, M.W., Kamo, N., Demura, M.
Organizer
16th International Conference on Retinal Proteins
Place of Presentation
長浜ロイヤルホテル(滋賀県)
Year and Date
2014-10-05 – 2014-10-10
Related Report
-
[Presentation] The importance of Thr218 for Cl--pumping photocycle of Natronomonas pharaonis halorhodopsin2014
Author(s)
Shibasaki, K., Shigemura, H., Kikukawa, T., Kamiya, M., Aizawa, T., Kamo, N., Demura, M.
Organizer
16th International Conference on Retinal Proteins
Place of Presentation
長浜ロイヤルホテル(滋賀県)
Year and Date
2014-10-05 – 2014-10-10
Related Report
-
[Presentation] Proton Transfer Reactions of Anabaena Sensory Rhodopsin2014
Author(s)
Hasemi, T., Kikukawa, T., Kamiya, M., Aizawa, T., Jung, K.H., Kamo, N., Demura, M.
Organizer
16th International Conference on Retinal Proteins
Place of Presentation
長浜ロイヤルホテル(滋賀県)
Year and Date
2014-10-05 – 2014-10-10
Related Report
-
-
-
-