| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Lysophoshatidic acid (LPA) is a lipid mediator and regulates various cellular responses via acting on LPA-specific G protein-coupled receptors (LPA1-6). We have previously found that mice deficient for PA-PLA1β, an LPA-producing enzyme, show abnormal structures in male organs including testes and vesicular glands. In this study, we aimed at identifying a LPA receptor that is involved in the PA-PLA1β-LPA axis in the male organogenesis. We also examined defects at a cellular level in the LPA-regulated male organs. We identified approximately a half of LPA6-deficient mice has smaller testes. We also found that mRNA for LPA6 is localized to the spermatogonium, Sertoli cells and Leydig cells in juvenile mice and that the expression decreases as mice become aged. Since the phenotype of PA-PLA1β-deficient mice and LPA6-deficient mice is different from a reported triple LPA1-3-deficient mice, LPA signal has at least two distinct roles in development of male organs.
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