Novel conjugation of the ubiquitin-like protein Atg8 and its biological significance

• Project/Area Number: 26440052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: OKAMOTO Noriko 大阪大学, 生命機能研究科, 特任講師(常勤) (90568750)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Koji 大阪大学, 生命機能研究科, 准教授 (40455217)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Atg8 / ユビキチン様タンパク質 / オートファジー / 翻訳後修飾 / タンパク質分解 / ミトコンドリア / 出芽酵母 / 膜ダイナミクス / 品質管理

Outline of Final Research Achievements

Atg8 is a phospholipid-conjugated ubiquitin-like protein essential for autophagy. Using budding yeast, this study demonstrated that Atg8 is also conjugated to several proteins under selective mitochondrial degradation-inducing conditions. In addition, Asu1 (Atg8ylation substrate 1), an endoplasmic reticulum membrane protein, has been identified as a substrate for Atg8 conjugation. It is possible that Atg8ylation contributes to autophagy-dependent degradation of Asu1.

Research Products

• [Journal Article] Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling (2015)
  • Author(s): Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K
  • Journal Title: EMBO Journal Volume: 34 Issue: 21 Pages: 2703-2719
  • DOI: 10.15252/embj.201591440
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Novel aspects of autophagy-related proteins under mitophagy-inducing conditions. (2014)
  • Author(s): 岡本 徳子
  • Organizer: 第66回 日本細胞生物学会大会
  • Place of Presentation: 奈良、奈良県新公会堂
  • Year and Date: 2014-06-11
  • Invited
• [Book] 実験医学 Close Up 実験法Vol.32 No.14 (9月号）2014 (2014)
  • Author(s): 田中敦、岡本徳子、風間智彦
  • Publisher: 羊土社
• [Remarks] 研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/okamoto/Okamoto_Lab/
• [Remarks] 研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/okamoto/Okamoto_Lab/toppu.html