The analysis of the molecular mechanism of amphibian metamorphosis using genomic editing

• Project/Area Number: 26440057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hiroshima University
• Principal Investigator: Yaoita Yoshio 広島大学, 両生類研究センター, 教授 (00166472)
• Co-Investigator(Kenkyū-buntansha): 田澤 一朗 広島大学, 両生類研究センター, 助教 (10304388) ; 中島 圭介 広島大学, 両生類研究センター, 助教 (60260311)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 変態 / 甲状腺ホルモン / 両生類 / げっ歯類 / 脳の変態 / 肝臓の変態 / 出生 / ネッタイツメガエル / 無尾両生類 / ゲノム編集 / 甲状腺ホルモン受容体 / TALEN / 細胞外基質分解酵素

Outline of Final Research Achievements

We have examined whether the birth of the mammalian newborn corresponds to metamorphosis. In rodent, the surge of thyroid hormone is observed in the first three postnatal weeks. Eleven genes up-regulated in the brain of Xenopus tropicalis during metamorphosis were analyzed in detail and their expression levels increased in the rodent brain during three postnatal weeks. The expression of six genes encoding proteins up-regulated in mouse brain during the three postnatal weeks was also induced in the tadpole brain during metamorphosis. These data indicate that the similar gene expression profile to that of metamorphosing tadpole brain was elicited in rodent brain and strongly suggested that the rodent brain undergoes metamorphosis.

Research Products

• [Int'l Joint Research] NIH/University of Virginia/ University of Rochester Medical Center(米国)
• [Int'l Joint Research] Dept of Microbiology & Immunology/University of Rochester/Medical Center(米国)
• [Int'l Joint Research] University of Rochester Medical Center/University of Virginia/university of Georgia(米国)
• [Journal Article] Thyroid hormone receptor α- and β-knockout Xenopus tropicalis tadpoles reveal subtype-specific roles during development. (2018)
  • Author(s): Keisuke Nakajima, Ichiro Tazawa and Yoshio Yaoita
  • Journal Title: Endocrinology Volume: 159 Issue: 2 Pages: 733-743
  • DOI: 10.1210/en.2017-00601
  • Peer Reviewed
• [Journal Article] Vitamin A induced homeotic hindlimb formation on dorsal and ventral sides of regenerating tissue of amputated tails of Japanese brown frog tadpoles. (2017)
  • Author(s): Ichiro Tazawa and Yoshio Yaoita
  • Journal Title: Development, Growth and Differentiation Volume: 59 Issue: 9 Pages: 688-700
  • DOI: 10.1111/dgd.12407
  • Peer Reviewed
• [Journal Article] Mechanisms of tail resorption during anuran metamorphosis (2017)
  • Author(s): Yuya Nakai, Keisuke Nakajima and Yoshio Yaoita
  • Journal Title: BioMolecular Concepts Volume: 8 Issue: 3-4 Pages: 179-183
  • DOI: 10.1515/bmc-2017-0022
  • Peer Reviewed
• [Journal Article] An inhibitor of thyroid hormone synthesis protects tail skin grafts transplanted to syngenic adult frogs. (2017)
  • Author(s): Yuya Nakai, Keisuke Nakajima and Yoshio Yaoita
  • Journal Title: Zoological Science Volume: 34 Issue: 5 Pages: 414-418
  • DOI: 10.2108/zs170011
  • NAID: 40021336199
  • Peer Reviewed
• [Journal Article] no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak Syndrome and allows visualization of internal organogenesis during tadpole development. (2017)
  • Author(s): Takuya Nakayama, Keisuke Nakajima, Amanda Cox, Marilyn Fisher, Mary Howell, Margaret B. Fish, Yoshio Yaoita, and Robert M. Grainger
  • Journal Title: Developmental Biology Volume: 426 Issue: 2 Pages: 472-486
  • DOI: 10.1016/j.ydbio.2016.08.020
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ouro proteins are not essential to tail regression during Xenopus tropicalis metamorphosis (2016)
  • Author(s): Y. Nakai, K. Nakajima, Jacques Robert and Y. Yaoita
  • Journal Title: Genes to Cells Volume: 21(3) Issue: 3 Pages: 275-286
  • DOI: 10.1111/gtc.12337
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Generation of albino Cynops pyrrhogaster by genomic editing of the tyrosinase gene (2016)
  • Author(s): Keisuke Nakajima, Taeko Nakajima, and Yoshio Yaoita
  • Journal Title: Zoological Science Volume: 33 Issue: 3 Pages: 290-294
  • DOI: 10.2108/zs150203
  • NAID: 210000164354
  • Peer Reviewed
• [Journal Article] Characterization of myosin II regulatory light chain isoforms in HeLa cells. (2015)
  • Author(s): Tomo Kondo, Morihiro Okada, Kayo Kunihiro, Masayuki Takahashi, Yoshio Yaoita, Hiroshi Hosoya, Kozue Hamao
  • Journal Title: Cytoskeleton (Hoboken) Volume: 72(12) Issue: 12 Pages: 609-620
  • DOI: 10.1002/cm.21268
  • Peer Reviewed
• [Journal Article] Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients (2015)
  • Author(s): Takuya Nakayama, Marilyn Fisher, Keisuke Nakajima, Akinleye O. Odeleye, Keith B. Zimmerman, Margaret B. Fish, Yoshio Yaoita, Jena L. Chojnowski, James D. Lauderdale, Peter A. Netland, Robert M. Grainger
  • Journal Title: Developmental Biology Volume: 408 Issue: 2 Pages: 328-344
  • DOI: 10.1016/j.ydbio.2015.02.012
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus (2015)
  • Author(s): K. Nakajima and Y. Yaoita
  • Journal Title: Biology Open Volume: 4(3) Issue: 3 Pages: 259-266
  • DOI: 10.1242/bio.201410926
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevisHighly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis (2015)
  • Author(s): K. Nakajima and Y. Yaoita
  • Journal Title: Biology Open Volume: 4(2) Issue: 2 Pages: 180-185
  • DOI: 10.1242/bio.201410009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Transformation during Amphibian Metamorphosis and Regeneration (2018)
  • Author(s): Yoshio Yaoita, Keisuke Nakajima and Ichiro Tazawa
  • Organizer: International Symposium at Hiroshima University: Amphibian development, regeneration, evolution and beyond
  • Int'l Joint Research / Invited
• [Presentation] Gene expression in anuran homeotic transformation from a tail to limbs (2018)
  • Author(s): Sho Morioka, Ichiro Tazawa and Yoshio Yaoita
  • Organizer: International Symposium at Hiroshima University: Amphibian development, regeneration, evolution and beyond
  • Int'l Joint Research
• [Presentation] Vitamin A-induced homeotic limb formation on dorsal and ventral sides of regenerating tissue of amputated tails of Japanese brown frog tadpoles (2018)
  • Author(s): Ichiro Tazawa and Yoshio Yaoita
  • Organizer: International Symposium at Hiroshima University: Amphibian development, regeneration, evolution and beyond
  • Int'l Joint Research
• [Presentation] ツメガエル精子MMP-2による早い電気的な多精防止 (2017)
  • Author(s): 平岩梓、渡部茉美、上野智代、上野秀一、中島圭介、矢尾板芳郎、岩尾康宏
  • Organizer: 第40回日本分子生物学会
• [Presentation] 「尾から肢が生えるふしぎ_」 (2017)
  • Author(s): 田澤 一朗, 森岡 晶, 矢尾板 芳郎
  • Organizer: 日本動物学会第８８回大会
  • Invited
• [Presentation] 無尾両生類の尾から肢への ホメオティック・トランスフォーメーションにおいて 過剰肢が生じる位置とその向き (2017)
  • Author(s): 田澤 一朗, 森岡 晶, 矢尾板 芳郎
  • Organizer: 第３回次世代両生類研究会
  • Invited
• [Presentation] ツメガエル受精時の多精拒否における精子 MMP-2 の役割 (2017)
  • Author(s): 平岩 梓、渡部 茉美、上野 智代、上野 秀一、中島 圭介、矢尾板 芳郎、岩尾 康宏
  • Organizer: 第88回日本動物学会
• [Presentation] 両生類変態期におけるキャスペースの働き (2017)
  • Author(s): 近藤綾香、中井裕也、中島圭介、矢尾板芳郎
  • Organizer: 第88回日本動物学会
• [Presentation] 甲状腺ホルモン受容体α、βの無尾両生類の変態期における異なる役割 (2017)
  • Author(s): 中島圭介、田澤一朗、矢尾板芳郎
  • Organizer: 第88回日本動物学会
• [Presentation] 甲状腺ホルモン受容体遺伝子のKOの解析によって明らかになったサブタイプの変態における役割 (2017)
  • Author(s): 中島圭介、田澤一朗、矢尾板芳郎
  • Organizer: 第１１回ツメガエル研究集会
• [Presentation] ヤマアカガエルの尾から肢へのホメオティックトランスフォーメーションにおける遺伝子発現解析 (2017)
  • Author(s): 森岡晶、田澤一朗、矢尾板芳郎
  • Organizer: 日本動物学会中国四国支部 広島県例会
• [Presentation] Expression of limb-development-related genes during the formation of homeotic limbs in Rana ornativentris. (2016)
  • Author(s): Sho Morioka, Ichiro Tazawa, and Yoshio Yaoita
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: Ginowan, Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Location and orientation of ectopic limbs and expression of genes related to limb development in tail-amputated and retinoid-treated tadpoles of Rana ornativentris. (2016)
  • Author(s): Ichiro Tazawa and Yoshio Yaoita
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: Ginowan, Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Ouro proteins are not essential to tail regression during Xenopus metamorphosis. (2016)
  • Author(s): Yoshio Yaoita, Yuya Nakai, Jacques Robert and Keisuke Nakajima
  • Organizer: 16th International Xenopus Conference
  • Place of Presentation: Chania (Crete), Greece
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] 無尾両生類のホメオティックトランスフォーメーションにおける Hox 遺伝子の発現解析 (2016)
  • Author(s): 森岡晶、田澤一朗、矢尾板芳郎
  • Organizer: 次世代両生類研究会第２回会合
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2016-08-08
• [Presentation] TALEN mRNAを注入した卵母細胞にホストトランスファー法を適用した高効率遺伝子破壊法 (2015)
  • Author(s): 中島圭介、矢尾板芳郎
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-01
• [Presentation] ツメガエルの変態における尾の退縮にouro蛋白質は関係していない (2015)
  • Author(s): 中井 裕也、中島 圭介、矢尾板 芳郎
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-01
• [Presentation] TALEN mRNAの卵母細胞への注射とhost transferによる高効率遺伝子破壊法 (2015)
  • Author(s): 中島圭介、矢尾板芳郎
  • Organizer: 第86回日本動物学会
  • Place of Presentation: 新潟市
  • Year and Date: 2015-09-17
• [Presentation] 無尾両生類ホメオティック肢の発生パターン (2015)
  • Author(s): 田澤 一朗, 矢尾板 芳郎
  • Organizer: 第86回日本動物学会大会
  • Place of Presentation: 新潟市
  • Year and Date: 2015-09-17
• [Presentation] TALEN法による高効率遺伝子破壊法：Xenopus laevis 卵母細胞へのmRNA注入とホストトランスファー法の応用 (2015)
  • Author(s): 中島圭介、矢尾板芳郎
  • Organizer: 第9回日本ツメガエル研究集会
  • Place of Presentation: 秋田市
  • Year and Date: 2015-09-14
• [Presentation] 致死遺伝子のノックアウト問題の解決を目指して： TALEN による生殖細胞優先的標的遺伝子破壊法の開発 (2014)
  • Author(s): 中島 圭介、矢尾板 芳郎
  • Organizer: 第９回 XCIJ-MA・第８回ツメガエル研究会ジョイント研究集会
  • Place of Presentation: 相模原市
  • Year and Date: 2014-11-28
• [Presentation] TALENを用いたネッタイツメガエル生殖細胞優先的ゲノム編集技術 (2014)
  • Author(s): 中島 圭介、矢尾板 芳郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] The role of the thyroid hormone receptor during Xenopus metamorphosis (2014)
  • Author(s): Keisuke Nakajima, Kenta Fujimoto and Yoshio Yaoita
  • Organizer: 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2014-11-07 – 2014-11-09
  • Invited
• [Presentation] ヤマアカガエルにおけるホメオティック肢形成過程 (2014)
  • Author(s): 田澤 一朗, 矢尾板 芳郎
  • Organizer: 日本動物学会
  • Place of Presentation: 仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ツメガエルにおける ouro 遺伝子ノックアウト (2014)
  • Author(s): 中井 裕也、中島 圭介、矢尾板 芳郎
  • Organizer: 日本動物学会
  • Place of Presentation: 仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] TALEN による生殖細胞特異的ゲノム編集法の開発 (2014)
  • Author(s): 中島 圭介、矢尾板 芳郎
  • Organizer: 日本動物学会
  • Place of Presentation: 仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] The development of TALEN methods to enhance the mutation efficiency and to perform genome editing preferentially in germ cells using Xenopus. (2014)
  • Author(s): Keisuke Nakajima and Yoshio Yaoita
  • Organizer: 15th International Xenopus Conference
  • Place of Presentation: Pacific Grove, CA, USA
  • Year and Date: 2014-08-24 – 2014-08-28
• [Remarks] 広島大学両生類研究センター 発生研究部門
  • URL: http://amphibian.hiroshima-u.ac.jp/members/embryology/