| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
It remained to be elucidated how the exogenous mRNA was recognized and degraded quickly. Here, we analyzed the mechanisms for quick degradation of in vitro synthesized mRNA and encephalomyocarditis virus RNA. Dom34-GTPBP forms the surveillance complex to eliminate the exogenous mRNAs. Exonuclease complex and RNaseL-OAS function to degrade the mRNAs quickly. Based on these findings, we searched for compounds to stabilize the in vitro synthesized mRNA through drug discovery high-throughput screening.
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