Quality control mechanisms for aberrant mRNA that emerges under stress conditions
| Project/Area Number |
26440059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya City University |
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Principal Investigator |
Hosoda Nao 名古屋市立大学, 大学院薬学研究科, 講師 (40438198)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | mRNA品質管理 / 翻訳 / mRNA分解 / エキソソーム / RNA品質管理 / エキソソーム複合体 / エキソヌクレアーゼ |
|---|
| Outline of Final Research Achievements |
It remained to be elucidated how the exogenous mRNA was recognized and degraded quickly. Here, we analyzed the mechanisms for quick degradation of in vitro synthesized mRNA and encephalomyocarditis virus RNA. Dom34-GTPBP forms the surveillance complex to eliminate the exogenous mRNAs. Exonuclease complex and RNaseL-OAS function to degrade the mRNAs quickly. Based on these findings, we searched for compounds to stabilize the in vitro synthesized mRNA through drug discovery high-throughput screening.
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Report
(4 results)
Research Products
(19 results)
