| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
This study measured the cellular response for the extracellular stimulus utilizing the controlled photo-cleavage of caged-serine under high speed imaging. By this measurement, it was revealed the intracellular signal would be performed by the propagation of the decrease in the concentration of CheY-P (signal molecule) and this propagation was performed due to the dephosphorylation of CheY-P by polar localized CheZ and diffusion of CheY-P molecules. By the simultaneous measurement of the time difference between two motors on the same cell and comparison it between wild-type and mutant cells, the dynamic change in CheY-P concentration directly regulates the rotational direction of motor in the absence of extracellular stimuli. The functional CheY-GFP fusion as the intracellular signal molecule could be constructed, however, the cell expressing this fusion unexpectedly exhibited repellent response to the excitation light for GFP. This problem should be untied to progress this study.
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