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Stractural and functional analyses on novel minor dyneins localized to the proximal regions in cilia and flagella

Research Project

Project/Area Number 26440074
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

Yagi Toshiki  県立広島大学, 生命環境学部, 教授 (40292833)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsダイニン / ATP / 微小管 / 鞭毛繊毛 / ATP / 鞭毛・繊毛 / 変異株
Outline of Final Research Achievements

A green algae Chlamydomonas has fifteen axonemal dynein heavy chains (DHCs), which hydrolyze ATP and generate force to translocate axonemal microtubules. We previously found that flagellar contents of four minor-type DHCs (DHC3, DHC4, DHC11, and DHC12) are 10-20% of other eleven major-type DHCs. We surmised that the four DHCs have some unique roles to produce flagellar bending movements. To understand the function of each minor-dynein, we isolated novel mutants missing specific DHCs and characterized the mutant motilities. Under usual culture condition, each mutant motility appeared to be comparable to that of wild type. Interestingly, however, the swimming speeds of mutants missing DHC4 or DHC12 decreased more steeply than that of wild type with the increase of viscosity of culture media, suggesting that the two DHCs generate more power than other DHCs to swim against large viscous resistance. This is the first report that reveals the function of minor dyneins.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results) Book (3 results)

  • [Int'l Joint Research] Emory University/University of Minnesota/UT Southwestern(米国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] 清華大学(中国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] X-ray fiber diffraction analysis shows dynamic changes in axial tubulin repeats in native microtubules depending on paclitaxel content, temperature and GTP-hydrolysis.2016

    • Author(s)
      Kamimura S, Fujita Y, Wada Y, Yagi T, Iwamoto H.
    • Journal Title

      Cytoskeleton (Hoboken)

      Volume: 73 Issue: 3 Pages: 131-44

    • DOI

      10.1002/cm.21283

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Axonemal dynein light chain-1 locates at the microtubule-binding domain of the γ heavy chain.2015

    • Author(s)
      Ichikawa M, Saito K, Yanagisawa HA, Yagi T, Kamiya R, Yamaguchi S, Yajima J, Kushida Y, Nakano K, Numata O and Toyoshima YY.
    • Journal Title

      Molicular Biology of the Cell

      Volume: 26 Issue: 23 Pages: 4236-47

    • DOI

      10.1091/mbc.e15-05-0289

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity.2014

    • Author(s)
      Oda T, Yanagisawa H, Yagi T, Kikkawa M.
    • Journal Title

      Journal of Cell Biology

      Volume: 204 Issue: 5 Pages: 807-819

    • DOI

      10.1083/jcb.201312014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.2014

    • Author(s)
      Ishikawa H, Ide T, Yagi T, Jiang X, Hirono M, Sasaki H, Yanagisawa H, Wemmer KA, Stainier DY, Qin H, Kamiya R, Marshall WF.
    • Journal Title

      Elife.

      Volume: 3 Pages: 1-24

    • DOI

      10.7554/elife.01566

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of the Microtubule-Binding Domain of Flagellar Dynein.2014

    • Author(s)
      KatoYS., Yagi T., Harris SA., Ohki S., Yura K., Shimizu Y., Honda S., Kamiya R., Burgess SA., Tanokura M.
    • Journal Title

      Structure

      Volume: 22 Pages: 1628-1638

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional diversity of axonemal dyneins as assessed by in vitro and in vivo motility assays of Chlamydomonas mutants.2014

    • Author(s)
      Kamiya R and Yagi T.
    • Journal Title

      Zoolog Sci.

      Volume: 31 Pages: 633-644

    • NAID

      40020231543

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Resurrcetion of flagellar bending movements in chlamydomonas pralyzed mutants at high pressure.2017

    • Author(s)
      Toshiki Yagi, and Masayoshi Nishiyama
    • Organizer
      Biophysical Society 61st Annual meeting
    • Place of Presentation
      New Orlreans, Lousiana, USA
    • Year and Date
      2017-02-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 軸糸直径サイズ変化による鞭毛繊毛の屈曲運動の制御2016

    • Author(s)
      八木俊樹、上村慎治、岩本裕之
    • Organizer
      第54回日本生物物理学会大会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Resurrection of flagellar bending movements in chlamydomonas paralyzed mutants at high pressure2016

    • Author(s)
      Toshiki Yagi, and Masayoshi Nishiyama
    • Organizer
      17th International Conference on the Cell & Molecular Biology of Chlamydomonas
    • Place of Presentation
      Kyoto International Converence Center
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 高圧負荷により誘導されるクラミドモナス非運動性変異株鞭毛の屈曲運動2015

    • Author(s)
      八木 俊樹, 西山 雅祥
    • Organizer
      第86回 日本動物学会大会
    • Place of Presentation
      新潟コンベンションセンター
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Axonemal dynein light chain-1 locates at the microtubule-binding domain of the γ heavy chain.2015

    • Author(s)
      Ichikawa M, Saito K, Yanagisawa HA, Yagi T, Kamiya R, Yamaguchi S, Yajima J, Kushida Y, Nakano K, Numata O and Toyoshima YY.
    • Organizer
      第53回 日本生物物理学会大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of high-pressure induced bending movements in Chlamydomonas paralyzed mutant flagella2014

    • Author(s)
      Yagi T. and Nishiyama M.
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Book] “Dyneins (2nd edition)” (King SM, editor) Chapter 7. PP. 219-249.2017

    • Author(s)
      Yagi Toshiki, Kamiya Ritsu.
    • Total Pages
      660
    • Publisher
      Elsevier, Amsterdam.
    • ISBN
      0128094710
    • Related Report
      2017 Annual Research Report
  • [Book] “Dyneins (2nd edition)” (King SM, editor)  chapter 9 Genetic approaches to axonemal dynein function in Chlamydomonas and other organisms.2017

    • Author(s)
      Yagi T. and Kamiya R.
    • Publisher
      Elsevier, Amsterdam.
    • Related Report
      2016 Research-status Report
  • [Book] “Handbook of dynein (2nd edition)” (K.Hirose & LA. Amos, editior) Chapter 13. Diversity of Chlamydomonas Axonemal Dyneins.2017

    • Author(s)
      Yagi T. and Kamiya R.
    • Publisher
      Pan Stanford Publishing Pte Ltd.
    • Related Report
      2016 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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