Noninvasive in vivo imaging using GFP and quantum dots for elucidation of cancer metastasis mechanism

• Project/Area Number: 26440075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Kita Sayaka 東京大学, 大学院理学系研究科(理学部), 特別研究員 (70343564)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: in vivoイメージング / 非侵襲 / ヒトがん細胞 / マウス / in vivo イメージング / がん細胞 / 乳がん細胞 / ゼノグラフトモデル / バイオチャンバー / GFP / 転移がん細胞 / がん幹細胞 / がん転移

Outline of Final Research Achievements

In this study, I succeeded in developing a system for non-invasively imaging the metastasis of cancer cells.
In addition, new knockout transgenic mice for noninvasive in vivo imaging targeted to metastatic cancer cells could be generated. It's transgenic mouse was generated using CRISPR / Cas9. The target gene was a Prkdc gene involved with T cells which are immune cells.

Research Products

• [Presentation] Noninvasive in vivo imaging of human cancer cells and mouse vascular endothelial cells in the auricle of mouse (2017)
  • Author(s): 喜多 清、樋口 秀男
  • Organizer: 第122回 日本解剖学会 総会全国学術集会
  • Place of Presentation: 長崎県 長崎市 長崎大学
  • Year and Date: 2017-03-28
• [Presentation] マウス耳介を用いたがん細胞および血管の非侵襲in vivoイメージング法の確立 (2017)
  • Author(s): 喜多 清, 樋口 秀男
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] Noninvasive in vivo imaging of human cancer cell and mouse macrophage in the auricle of mouse (2016)
  • Author(s): Sayaka Kita and Hideo Higuchi
  • Organizer: 第121回日本解剖学会総会
  • Place of Presentation: 福島県郡山市 (ビックパレットふくしま)
  • Year and Date: 2016-03-28
• [Presentation] Novel mouse xenograft model for noninvasive in vivo imaging of human tumor cell and tissue in the auricle (2015)
  • Author(s): 喜多 清、樋口 秀男
  • Organizer: 第120回 日本解剖学会総会全国学術集会
  • Place of Presentation: 兵庫県 神戸市 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Novel mouse xenograft model for noninvasive in vivo imaging of human tumor cell and tissue in the auricle (2014)
  • Author(s): Sayaka Kita and Hideo Higuch
  • Organizer: The 2014 Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia, Pennsylvania, USA,
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Noninvasive in vivo imaging of tumor cells and tissue in mouse auricles (2014)
  • Author(s): Sayaka Kita and Hideo Higuch
  • Organizer: 第52回 日本生物物理学会年会
  • Place of Presentation: 北海道 札幌市 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27