| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Molecular motors in living organisms work with the hydrolysis energy of nucleotide substrates. In fact, with regard to ATP-driven molecular motors, it is thought that they move by sequentially generating force according to each chemical state such as ATP, ADP・Pi, ADP, Pi. Here, I tried to measure at single-molecule level potential energies that are viewed as the design drawing of the force generation. Specifically, using a rotary molecular motor F1-ATPase, the rotation was manipulated with magnetic tweezers while directly observing the bound nucleotides. Torque in a chemical state of one ADP binding was measured and the potential energy was obtained from the integration of torque.
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