| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
CADM1, identified as an adhesion molecule in pancreatic islet alpha cells, has been reported not only to communicate among alpha cells and between nerve fibers. First, we investigated the effect of CADM1 expression on the movement of intracellular secretory granules in alpha cells. A mean velocity of granules in alpha cells that expressed CADM1 endogenously was significantly decreased in CADM1-knockdown cells. Next, we have visualized glucagon secretion using a method of video-rate bioluminescence imaging. The fusion protein of proglucagon and Gaussia luciferase was used as a reporter to detect glucagon secretion and was efficiently expressed in pancreatic alpha cells. The secretory events were observed frequently at the intercellular contact regions in response to stimulation by KCl. These results suggested that the cell-cell adhesion strongly affected the intracellular movement and the fusion with plasma membrane of intracellular granules.
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