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The interlocked rotation between two rotary molecular motors in ATP synthase VoV1 -The mechanism of intermolecular torque transmission-

Research Project

Project/Area Number 26440084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionOsaka Medical College

Principal Investigator

FURUIKE Shou  大阪医科大学, 医学部, 講師 (60392875)

Co-Investigator(Renkei-kenkyūsha) YOKOYAMA Ken  京都産業大学, 総合生命科学部, 教授 (70271377)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsATP合成酵素 / 分子モーター / 1分子観察 / 1分子観察
Outline of Final Research Achievements

ATP synthase VoV1 (or FoF1) is composed of two rotary molecular motors. The V1 (Vo) motor synthesizes/hydrolyzes ATPs, and the Vo (Fo) motor transmits/transports protons. Because the two motors are connected together by a common rotary shaft, the rotational angles and the functions of two motors also should be tightly coupled each together. To elucidate the couplings, we examined by single molecule observation, how is the torque transmission between the two motors affected by a torsional/bending rigidity of the rotary shaft. We constructed F1 mutants in which the shafts would retain nearly genuine shape and have weak torsional/bending rigidity. The rotation rates with and without drag were ~70% and almost same of wild type, respectively.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Direct observation of DNA overwinding by reverse gyrase2015

    • Author(s)
      Ogawa T, Yogo K, Furuike S, Sutoh K, Kikuchi A, Kinosita K Jr.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 112 Issue: 24 Pages: 7495-7500

    • DOI

      10.1073/pnas.1422203112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] None of the rotor residues of Fl-ATPase are essential for torque generation2014

    • Author(s)
      Ryohei Chiwata, Ayako Kohori, Tomonari Kawakami, Katsuyuki Shiroguchi, Shou Furuike, Kengo Adachi, Kazuo Sutoh, Masasuke Yoshida, and Kazuhiko Kinosita, Jr
    • Journal Title

      Biophys. J.

      Volume: 106 Issue: 10 Pages: 2166-2174

    • DOI

      10.1016/j.bpj.2014.04.013

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 自由連結部位で繋がれた回転シャフトを持つF1-ATPase の回転2018

    • Author(s)
      古池晶, 曽我直樹, 牧泰史, 吉田秀司
    • Organizer
      日本物理学会 第73回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Single α-helix alone in a shaft of F1-ATPase cannot fully transmit the torque but closely cooperate with the stator2017

    • Author(s)
      Shou Furuike, Yasushi Maki, Hideji Yoshida
    • Organizer
      日本生物物理学会 第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 回転軸の長さを半分にしたF1-ATPaseの回転とATP加水分解素過程との連動2016

    • Author(s)
      遠藤愛子, 牧泰史, 吉田秀司, 横山謙, 古池晶
    • Organizer
      日本物理学会 第71回年次大会
    • Place of Presentation
      東北学院大学 仙台市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Direct Observation of DNA Overwinding by Reverse Gyrase2016

    • Author(s)
      Ogawa T, Yogo K, Furuike S, Sutoh K, Kikuchi A, Kinosita K Jr.
    • Organizer
      Biophysical society 60th Annual Meeting
    • Place of Presentation
      Los Angeles Convention Center, Los Angeles, California
    • Year and Date
      2016-02-29
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Rotor function of flagella protein FliJ in A3B3 of V1-ATPase2015

    • Author(s)
      Baba M, Nakanishi A, Kishikawa J, Furuike S, Yokoyama K
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学 金沢市
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
  • [Presentation] Direct observation of DNA overwinding by reverse gyrase2015

    • Author(s)
      Ogawa T, Yogo K, Furuike S, Sutoh K, Kikuchi A, Kinosita K Jr.
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学 金沢市
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] Reverse gyrase likely biases thermal DNA strand passage toward overwinding2014

    • Author(s)
      T. Ogawa, Katsunori Yogo, S. Furuike, K. Sutoh, A. Kikuchi, K. Kinosita.
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター 札幌市
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] The ingenious structure of central rotor apparatus in VoV1; torque transmission mechanism in the central rotor of VoV12014

    • Author(s)
      A. Nakanishi, J. Kishikawa, M. Tamakoshi, S. Furuike, K. Yokoyama.
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター 札幌市
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Torque generation mechanism in V1 motor2014

    • Author(s)
      M. Baba, S. Furuike, A. Nakanishi, J. Kishikawa, N. Takeuchi, K. Yokoyama
    • Organizer
      日本生物物理学会
    • Place of Presentation
      札幌コンベンションセンター 札幌市
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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