Development of direct observation system for cell-to-cell communications via humoral factors based on the single cell real-time secretion assay.
| Project/Area Number |
26440087
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | The University of Tokyo (2015-2016)
The Institute of Physical and Chemical Research (2014) |
|---|
Principal Investigator |
Yamagishi Mai 東京大学, 大学院理学系研究科(理学部), 特任研究員 (90332501)
|
|---|
| Research Collaborator |
SHIRASAKI YOSHITAKA 東京大学, 大学院理学系研究科, 助教
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 一細胞 / イメージング / 分泌 / 細胞間相互作用 / 生細胞リアルタイムイメージング / 要素技術開発 / サイトカイン / エクソソーム / イメージングシステム |
|---|
| Outline of Final Research Achievements |
In this study, to understand biological systems by directly analyzing cell-to-cell communication network dynamics, I tried to develop the following systems. That is, a platform for visualizing humoral factors secreted from individual cells with interacting each other, and a method that can quantify gene expression in the cells on the platform. I successfully developed a prototype of the platform that allowed to detect secretion with maintaining cell-to-cell communication, and actually confirmed that secretion detection could be achieved at a single cell level. Beside, a system for RNA-FISH on the platform was established. Furthermore, I also launched additional challenges including real-time monitoring of mRNA expression as a visualization method for cell responses, visualization of exosome release from a single cell, and so on.
|
Report
(4 results)
Research Products
(14 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] High-throughput single-cell secretion measurement on an optical waveguide chip.2014
Author(s)
Shirasaki, Y., Suzuki, N., Yamagishi, M., Nakahara, A., Shoji, S. and Ohara, O.
Organizer
The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Presentation
Henry B. Gonzalez Convention Center, San Antonio, Texas, USA
Year and Date
2014-10-26 – 2014-10-30
Related Report
