| Project/Area Number |
26440087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo (2015-2016)
The Institute of Physical and Chemical Research (2014) |
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Principal Investigator |
Yamagishi Mai 東京大学, 大学院理学系研究科(理学部), 特任研究員 (90332501)
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| Research Collaborator |
SHIRASAKI YOSHITAKA 東京大学, 大学院理学系研究科, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 一細胞 / イメージング / 分泌 / 細胞間相互作用 / 生細胞リアルタイムイメージング / 要素技術開発 / サイトカイン / エクソソーム / イメージングシステム |
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| Outline of Final Research Achievements |
In this study, to understand biological systems by directly analyzing cell-to-cell communication network dynamics, I tried to develop the following systems. That is, a platform for visualizing humoral factors secreted from individual cells with interacting each other, and a method that can quantify gene expression in the cells on the platform. I successfully developed a prototype of the platform that allowed to detect secretion with maintaining cell-to-cell communication, and actually confirmed that secretion detection could be achieved at a single cell level. Beside, a system for RNA-FISH on the platform was established. Furthermore, I also launched additional challenges including real-time monitoring of mRNA expression as a visualization method for cell responses, visualization of exosome release from a single cell, and so on.
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