Motility of eukaryotic flagella studied with the bottom-up strategy
| Project/Area Number |
26440089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
Oiwa Kazuhiro 国立研究開発法人情報通信研究機構, 未来ICT研究所, 主管研究員 (10211096)
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| Research Collaborator |
KIRIMA Junya
SHIRAGA Misaki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ダイニン / 鞭毛運動 / 再構成実験系 / 自己組織化 / 鞭毛・繊毛運動 / 試験管内再構成実験 / 鞭毛・繊毛 / 細胞運動 |
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| Outline of Final Research Achievements |
The complexity of the eukaryotic flagellar axoneme is derived from 200-600 types of modular building blocks assembled hierarchically. These building blocks are precisely arranged along doublet microtubules(MTs) and regulated in a coordinated fashion to produce periodic flagellar beating. To reveal the mechanism, we have carried out in vitro reconstitution of axonemal structures in a bottom-up manner. To MTs polymerized from fragmented axonemes, we added crude outer-arm dynein extract from Chlamydomonas axonemes. These dyneins formed regular arrays on the MTs in the self-organized manner and stiff MT-bundles. Addition of ATP occasionally elicited association and dissociation cycles of MTs. When both ends of a MT bundle were clamped, shear between MTs at the middle part forced MTs to loop out from the bundle and the characteristic S-shaped bending was formed. These cyclic interactions between dynein and MTs will be an essence of the beating mechanism of the axoneme.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Creating biomolecular motors based on dynein and actin-binding proteins.2017
Author(s)
Furuta, A., Amino, M., Yoshio, M., Oiwa, K., Kojima, H., and Furuta, K.
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Journal Title
Nature Nanotechnology
Volume: 12
Issue: 3
Pages: 233-237
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics2017
Author(s)
Mingyue Jin, Oz Pomp, Tomoyasu Shinoda, Shiori Toba, Takayuki Torisawa, Ken’ya Furuta, Kazuhiro Oiwa, Takuo Yasunaga, Daiju Kitagawa, Shigeru Matsumura, Takaki Miyata, Thong Teck Tan, Bruno Reversade & Shinji Hirotsune
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 39902-39902
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein2014
Author(s)
Torisawa, T., Ichikawa, M., Furuta, A., Saito, K., Oiwa, K., Kojima, H., Toyoshima, Y. Y. and Furuta, K.
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Journal Title
Nature Cell Biol
Volume: 16
Issue: 11
Pages: 1118-1118
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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