| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
The complexity of the eukaryotic flagellar axoneme is derived from 200-600 types of modular building blocks assembled hierarchically. These building blocks are precisely arranged along doublet microtubules(MTs) and regulated in a coordinated fashion to produce periodic flagellar beating. To reveal the mechanism, we have carried out in vitro reconstitution of axonemal structures in a bottom-up manner. To MTs polymerized from fragmented axonemes, we added crude outer-arm dynein extract from Chlamydomonas axonemes. These dyneins formed regular arrays on the MTs in the self-organized manner and stiff MT-bundles. Addition of ATP occasionally elicited association and dissociation cycles of MTs. When both ends of a MT bundle were clamped, shear between MTs at the middle part forced MTs to loop out from the bundle and the characteristic S-shaped bending was formed. These cyclic interactions between dynein and MTs will be an essence of the beating mechanism of the axoneme.
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