| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
To investigate the spatial patterning mechanisms of actin filaments, microtubules, intermediate filaments and focal adhesions, these spatial relationships in a diffraction-limited area were analyzed by multitarget super-resolution microscopy, IRIS. These multiplexed super-resolution images showed area-specific proximity between cytoskeletons and focal adhesions. The combination of IRIS with live-cell imaging of microtubule tips showed that the speed and direction of microtubule growth is highly influenced by collision and subsequent interaction with actin stress fibers. These results suggest that the formation process of cytoskeletal structures dynamically interact with each other.
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