Study of the structural and functional diversities of vertebrate cilia

• Project/Area Number: 26440094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: University of Yamanashi
• Principal Investigator: NARITA Keishi 山梨大学, 総合研究部, 准教授 (50452131)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 繊毛 / 軸糸 / 多様性 / プロテオーム / ゲノム編集 / 構造解析 / TRPV4 / 血液脳脊髄液関門 / 上皮細胞 / 極性輸送

Outline of Final Research Achievements

A novel gene involved in the proper formation of vertebrate cilia was identified and characterized. The knockout mouse demonstrated a severe phenotype after birth due to defects in ciliogenesis. Transmission electron microscopy of the affected organs revealed that the integrity of axonemal arrangement and number were lost in the null mutant. Although there is no clinical report related to this gene, SNP analysis suggested the existence of SNPs that would lead to the loss of function of this gene.

Research Products

• [Journal Article] 9 + 0 and 9 + 2 cilia are randomly dispersed in the mouse node (2016)
  • Author(s): Odate T, Takeda S, Narita K, Kawahara T.
  • Journal Title: Microscopy (Oxf) Volume: 65 Issue: 2 Pages: 119
  • DOI: 10.1093/jmicro/dfv352
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Observation of the ciliary movement of choroid plexus epithelial cells Ex vivo (2015)
  • Author(s): Inoue T, Narita K, Nonami Y, Nakamura H, Takeda S.
  • Journal Title: Journal of visual experiment Volume: 2015 Issue: 101 Pages: 572-584
  • DOI: 10.3791/52991
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cilia in the choroid plexus: Their roles in hydrocephalus and beyond (2015)
  • Author(s): Narita K, Takeda S.
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 9 Issue: FEB Pages: 5
  • DOI: 10.3389/fncel.2015.00039
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TRPV4 regulates the integrity of the blood-cerebrospinal fluid barrier and modulates transepithelial protein transport (2015)
  • Author(s): Narita K, Sasamoto S, Koizumi S, Okazaki S, Nakamura H, Inoue T, Takeda S.
  • Journal Title: FASEB Journal Volume: 29 Issue: 6 Pages: 2247
  • DOI: 10.1096/fj.14-261396
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 新規繊毛関連遺伝子ノックアウトマウスの解析 (2017)
  • Author(s): 成田啓之、長友啓明、竹田扇
  • Organizer: 第112回日本解剖学会学術集会
  • Place of Presentation: 長崎大学坂本キャンパス（長崎県長崎市）
• [Presentation] 山梨大学における肉眼解剖学実習の現状と課題 (2017)
  • Author(s): 成田啓之、竹田扇
  • Organizer: 第112回日本解剖学会学術集会
  • Place of Presentation: 長崎大学坂本キャンパス（長崎県長崎市）
• [Presentation] 新規繊毛関連遺伝子ノックアウトマウスの表現型解析 (2016)
  • Author(s): 成田啓之、長友啓明、若山照彦、竹田扇
  • Organizer: 第76回日本解剖学会中部支部学術集会
  • Place of Presentation: 信州大学医学部（長野県松本市）
  • Year and Date: 2016-10-08
• [Presentation] 新規繊毛関連遺伝子の機能解析 (2016)
  • Author(s): Narita K, Nagatomo H, Wakayama T, Takeda S
  • Organizer: 日本解剖学会
  • Place of Presentation: ビッグパレット福島
  • Year and Date: 2016-03-25
• [Presentation] Regulation of the Blood-Cerebrospinal Fluid Barrier Functions by TRPV4. (2015)
  • Author(s): Narita K, Sasamoto S, Koizumi S, Okazaki S, Nakamura H, Inoue T, Takeda S.
  • Organizer: 日本神経科学学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-07-28
• [Presentation] Regulation of the blood-cerebrospinal fluid barrier permeability by TRPV4 (2015)
  • Author(s): Narita K, Sasamoto S, Koizumi S, Okazaki S, Nakamura H, Inoue T, Takeda S.
  • Organizer: 日本解剖学会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] TRPV4による血液脳脊髄液関門の機能調節 (2014)
  • Author(s): 成田啓之、 笹本祥平、 小泉修一、 竹田扇
  • Organizer: 日本解剖学会中部地方会
  • Place of Presentation: 金沢大学宝町キャンパス（石川県金沢市）
  • Year and Date: 2014-10-11 – 2014-10-12