The role of septin participating in the remodeling of neuronal circuits

• Project/Area Number: 26440095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: Ageta-Ishihara Natsumi 名古屋大学, 理学研究科, 講師 (60547561)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 細胞骨格 / 学習 / 記憶 / 神経

Outline of Final Research Achievements

Cytoskeletal remodeling in response to synaptic inputs is a critical process underlying structural plasticity, as is exemplified by the dependence of late-phase LTP and long-term memory on actin remodeling. Unbiased behavioral screening of several mouse lines that are deficient in or overloaded with a septin subunit or a related protein revealed that mice of one such line consistently underperform spatial learning tasks. The unique line may provide a clue to unknown molecular mechanism of spatial cognition and learning. Proteomic analysis revealed quantitative changes in the glutamate receptor subunits and other proteins in the hippocampus.

Research Products

• [Journal Article] Methods for Immunoblot Detection and High-resolution Subcellular Mapping of Septin (2016)
  • Author(s): Parajuli L, Ageta-Ishihara N, Ageta H, Fukazawa Y, Kinoshita M.
  • Journal Title: Methods in Cell Biology Volume: 136
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization. (2016)
  • Author(s): Horigane S, Ageta-Ishihara N, Kamijo S, Fujii H, Okamura M, Kinoshita M, Takemoto-Kimura S, Bito H
  • Journal Title: Mol Brain. Volume: 9 Issue: 1 Pages: 8-8
  • DOI: 10.1186/s13041-016-0189-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance. (2015)
  • Author(s): Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka T, Kaibuchi K, Hattori S, Miyakawa T, Tanaka K, Huda F, Hirai H, Hashimoto K, Watanabe M, Sakimura K, Kinoshita M.
  • Journal Title: Nat Commun. Volume: 6 Issue: 1 Pages: 10090-10090
  • DOI: 10.1038/ncomms10090
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transgenic supplementation of SIRT1 fails to alleviate acute loss of nigrostriatal dopamine neurons and gliosis in a mouse model of MPTP-induced parkinsonism. (2015)
  • Author(s): Kitao Y, Ageta-Ishihara N, Takahashi R, Kinoshita M, Hori O.
  • Journal Title: F1000Res. Volume: 4 Pages: 130-130
  • DOI: 10.12688/f1000research.6386.1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] SIRT1 overexpression ameliorates a mouse model of SOD1-linked amyotrophic lateral sclerosis via HSF1/HSP70i chaperone system. (2014)
  • Author(s): Watanabe S, Ageta-Ishihara N, Nagatsu S, Takao K, Komine O, Endo F, Miyakawa T, Misawa H, Takahashi R, Kinoshita M, Yamanaka K.
  • Journal Title: Mol Brain Volume: 7 Issue: 1 Pages: 62-62
  • DOI: 10.1186/s13041-014-0062-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A FIB-SEM-based Method for High-Resolution Measurement of Morphological Parameters of Axospinous Synapses. (2017)
  • Author(s): Elhanbaly E, Ishikawa T, Ageta-Ishihara N, Kinoshita M, Fukazawa Y.
  • Organizer: The 1st ABiS Symposium
  • Place of Presentation: 生理学研究所（愛知県）
  • Year and Date: 2017-02-20
  • Int'l Joint Research / Invited
• [Presentation] Immunoelectron Microscopy 3D Reconstruction Analysis of Septin Subunits in the Cerebellum (2017)
  • Author(s): Kurita H, Fukazaw Y, Ageta-Ishihara N, Shigemoto R, Kinoshita M.
  • Organizer: The 1st ABiS Symposium
  • Place of Presentation: 生理学研究所（愛知県）
  • Year and Date: 2017-02-19
• [Presentation] Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation (2016)
  • Author(s): Ageta-Ishihara N, Miyata T, Watanabe M, Bito H, Kinoshita M
  • Organizer: 第39回神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2016-07-22
  • Invited
• [Presentation] Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation (2016)
  • Author(s): Ageta-Ishihara N, Miyata T, Watanabe M, Bito H, Kinoshita M
  • Organizer: 第39回神経科学学会 シンポジウム
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2016-07-22
  • Invited
• [Presentation] Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization. (2016)
  • Author(s): Horigane S, Ageta-Ishihara N, Kamijo S, Fujii H, Okamura M, Kinoshita M, Takemoto-Kimura S, Bito H.
  • Organizer: 第39回神経科学学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2016-07-20
• [Presentation] Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization (2016)
  • Author(s): Horigane S, Ageta-Ishihara, Kamijo S, Fujii H, Okamura M, Kinoshita M, Takemoto-Kimura S, Bito H.
  • Organizer: 第39回神経科学学会 口頭
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2016-07-20
• [Presentation] Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization (2016)
  • Author(s): Horigane S, Ageta-Ishihara, Kamijo S, Fujii H, Okamura M, Kinoshita M, Takemoto-Kimura S, Bito H.
  • Organizer: 第16回東京大学生命科学シンポジウム
  • Place of Presentation: 東京大学駒場キャンパスKOMCEE21(東京都）
  • Year and Date: 2016-04-23
• [Presentation] Spatial discrimination defects caused by chronic, systemic loss of a septin subunit is recapitulated by its acute, local depletion in the hippocampal dentate gyrus (2016)
  • Author(s): Ageta-Ishihara N, Yamada T, Suzuki Y, Sawada A, Masuda H, Takao K, Miyakawa T, Hioki H, Okamura M, Bito H, Inokuchi K, Kinoshita M
  • Organizer: 包括脳適応回路シンポジウム
  • Place of Presentation: 同志社大学 室町キャンパス 寒梅館 ハーディーホール（京都市）
  • Year and Date: 2016-03-03
• [Presentation] Analysis of septin mutant mice with impaired spatial recognition (2015)
  • Author(s): Ageta-Ishihara N、Masuda H、Mano Y、Sawada A、Yano Y、Takao K、Miyakawa T、Shigemoto S、Fukazawa Y、Kinoshita M
  • Organizer: 第38回神経科学学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-07-29
• [Presentation] シナプスを包囲するグリア膜直下のCDC42EP4/septin複合体はグルタミン酸クリアランスの足場となる (2015)
  • Author(s): 上田(石原)奈津実、山崎真弥、今野幸太郎、中山寿子、阿部学、橋本謙二、西岡朋生、貝淵弘
  • Organizer: 第88回日本薬理学会年会, シンポジウム
  • Place of Presentation: 名古屋国際会議場（愛知県）
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] CDC42EP4/septin-based perisynaptic glial scaffold that regulates glutamate clearance. (2015)
  • Author(s): Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Miyakawa T, Hashimoto K, Watanabe M, Sakimura K, Kinoshita M.
  • Organizer: The 18th iCeMS International Symposium / The15th International Membrane Research Forum
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2015-03-03
• [Presentation] CDC42EP4-septin complex in parasynaptic domains of Bergmann glia regulates cerebellar motor learning via GLAST-mediated glutamate clearance from the parallel fiber-Purkinje cell synapses. (2015)
  • Author(s): Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Miyakawa T, Hashimoto K,
  • Organizer: Gordon Research Conference “Glial Biology: Functional Interactions Among Glia & Neurons”
  • Place of Presentation: Four Points Sheraton / Holiday Inn Express（Ventura, CA, USA）
  • Year and Date: 2015-03-02 – 2015-03-04
• [Presentation] Filling the gap between genetic and behaviors traits at ultrastructural level. (2015)
  • Author(s): Ageta-Ishihara N, Masuda H, Mano Y, Yano Y, Fukazawa Y, Kinoshita M.
  • Organizer: IGER International Symposium“Frontiers in Biological Research with Advanced Electron Microscope Technologies”
  • Place of Presentation: 名古屋大学（愛知県)
  • Year and Date: 2015-01-15
  • Invited
• [Presentation] CDC42EP4-septin complex in parasynaptic domains of Bergmann glia facilitates cerebellar motor learning via GLAST-mediated glutamate clearance from the parallel fiber-Purkinje cell synapses (2014)
  • Author(s): Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Miyakawa T,
  • Organizer: 第44回北米神経科学学会年会
  • Place of Presentation: Washington Convention Center(Washington, DC )
  • Year and Date: 2014-11-17
• [Presentation] CDC42EP4-septin complex facilitates glutamate clearance from the parallel fiber-Purkinje cell synapses by concentrating GLAST to perisynaptic membrane domains of Bergmann glia (2014)
  • Author(s): 上田(石原)奈津実、山崎真弥、今野幸太郎、中山寿子、阿部学、橋本謙二、西岡朋生、貝淵弘三、宮川剛、橋本浩一、渡辺雅彦、﨑村建司、木下 専
  • Organizer: 第57回日本神経化学会大会、第57回日本生物学的精神医学会合同年会
  • Place of Presentation: 奈良県文化会館(奈良県)
  • Year and Date: 2014-09-29
  • Invited
• [Presentation] CDC42EP4/septin-dependent localization of GLAST to parasynaptic domains of Bergmann (2014)
  • Author(s): Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka N,
  • Organizer: 第37回日本神経科学大会, シンポジウム
  • Place of Presentation: パシフィコ横浜（神奈川県)
  • Year and Date: 2014-09-13
  • Invited