| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
We revealed that yorkie (yki) mRNA is localized to the P-bodies, which is ribonucleoprotein complexes for translational repression and exist adjacent to the ER, depending on a secondary RNA structure in its own 3’ untranslated region (UTR). Furthermore, we showed that the transgenic flies expressing the yorkie mRNA deleted the secondary RNA structure exhibited the enlarged compound eye phenotype. Further analyses using this fly line indicated that the secondary RNA structure in yki 3’UTR has an important role for translational repression in vivo. For the regulation of yki functional expression, importance of post-translational regulation of Yorkie by phosphorylation has been well established. However, in this study, we suggested another novel regulation which has similarly strong impact on yki gene expression.
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