Molecular mechanism and biological function of yki mRNA foci formation in Drosophila
Project/Area Number |
26440097
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
Yoshida Hideki 京都工芸繊維大学, 応用生物学系, 助教 (30570600)
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Research Collaborator |
UMEGAWACHI Takanari
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | yorkie / mRNAの小胞体標的化 / ショウジョウバエ / SRP非依存的 / 翻訳抑制 / ステムループ / 小胞体標的化RNA / SRP非依存的標的化 / yki mRNA |
Outline of Final Research Achievements |
We revealed that yorkie (yki) mRNA is localized to the P-bodies, which is ribonucleoprotein complexes for translational repression and exist adjacent to the ER, depending on a secondary RNA structure in its own 3’ untranslated region (UTR). Furthermore, we showed that the transgenic flies expressing the yorkie mRNA deleted the secondary RNA structure exhibited the enlarged compound eye phenotype. Further analyses using this fly line indicated that the secondary RNA structure in yki 3’UTR has an important role for translational repression in vivo. For the regulation of yki functional expression, importance of post-translational regulation of Yorkie by phosphorylation has been well established. However, in this study, we suggested another novel regulation which has similarly strong impact on yki gene expression.
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] ④Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.2016
Author(s)
Moyu Goto, Narumi Toda, Kouhei Shimaji, Dang Ngoc-Anh Suong, Nicole Vo, Hiroshi Kimura, Hideki Yoshida, Yoshihiro H. Inoue and Masamitsu Yamaguchi.
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Journal Title
Spermatogenesis.
Volume: 6
Issue: 3
Pages: e1232023-e1232023
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing2015
Author(s)
Miki Yamamoto-Hino, Hideki Yoshida, Tomomi Ichimuya, Sho Sakamura, Megumi Maeda, Yoshinobu Kimura, Norihiko Sasaki, Kiyoko F. Aoki-Kinoshita, Akiko Kinoshita-Toyoda, Hidenao Toyoda, Ryu Ueda, Shoko Nishihara, Satoshi Goto
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Journal Title
Genes to Cells
Volume: 20
Issue: 6
Pages: 521-542
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Knockdown of the drosophila FIG4 induces deficient locomotive behavior, shortening of motor neuron, axonal targeting aberration, reduction of life span and defects in eye development2015
Author(s)
Kyotani A, Azuma Y, Yamamoto I, Yoshida H, Mizuta I, Mizuno T, Nakagawa M, Tokuda T, Yamaguchi M
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Journal Title
Exp Neurol
Volume: 277
Pages: 86-95
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.2014
Author(s)
Yanai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
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Journal Title
Oncogenesis
Volume: 3
Issue: 4
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Subcellular localization of yki mRNAs is regulated by stem-loop in the 3’UTR2016
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tetsuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Krause M. Henry, Hideki Yoshida
Organizer
RNA 2016
Place of Presentation
国立京都国際会館(京都府・京都市)
Year and Date
2016-06-28
Related Report
Int'l Joint Research
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[Presentation] A genome-wide screen for mRNAs targeting the ER in SRP-independent manner, and analysis on subcellular localization of yki mRNA2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Hideki Yoshida
Organizer
第37回 日本分子生物学会年会
Place of Presentation
パシフィコ横浜(横浜)
Year and Date
2014-11-25 – 2014-11-27
Related Report
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[Presentation] Identification of non-canonical mRNAs targeting to the ER and analysis on subcellular localization of mRNAs by RNA imaging system2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Henry M. Krause, Hideki Yoshida
Organizer
The Nineteenth Annual Meeting of the RNA Society
Place of Presentation
Quebec city convention centre(ケベックシティ)
Year and Date
2014-06-03 – 2014-06-08
Related Report
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