Molecular basis of nuclear structures regulated by nuclear pore complex proteins

• Project/Area Number: 26440098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: ASAKAWA Haruhiko 大阪大学, 生命機能研究科, 准教授 (70399533)
• Research Collaborator: HIRAOKA Yasushi ; ZHANG Xu
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 核膜孔複合体 / 核構造 / 分裂酵母 / 核膜 / 細胞生物学

Outline of Final Research Achievements

We found that a non-essential nuclear pore complex protein Nup132 is essential for establishment of kinetochore structure and for attachment of kinetochore and microtubule in the meiotic first division, firstly indicating that the nuclear pore complex participates in the meiotic chromosome segregation. Next, we revealed a specific but dispensable regulation for expressions of nuclear pore complex proteins Nup98 and Nup96. We also found that an inner nuclear membrane protein Lem2 is essential for centromere-specific heterochromatin formation. In addition, we developed a novel fluorescent probe to visualize histone modification.

Research Products

• [Journal Article] Spatial organization of the Schizosaccharomyces pombe genome within the nucleus. (2017)
  • Author(s): Matsuda A, Asakawa H, Haraguchi T, Hiraoka Y.
  • Journal Title: Yeast. Volume: 34 Issue: 2 Pages: 55-66
  • DOI: 10.1002/yea.3217
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A genetically encoded probe for live-cell imaging of H4K20 monomethylation (2016)
  • Author(s): Sato Y., Kujirai T., Arai R., Asakawa H., Ohtsuki C., Horikoshi N., Yamagata K., Ueda J., Nagase T., Haraguchi T., Hiraoka Y., Kimura A., Kurumizaka H., Kimura, H.
  • Journal Title: J. Mol. Biol. Volume: 428 Issue: 20 Pages: 3885-3902
  • DOI: 10.1016/j.jmb.2016.08.010
  • Peer Reviewed / Open Access
• [Journal Article] Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions. (2016)
  • Author(s): Tange Y, Chikashige Y, Takahata S, Kawakami K, Higashi M, Mori C, Kojidani T, Hirano Y, Asakawa H, Murakami Y, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes to Cells Volume: 21 Issue: 8 Pages: 812-832
  • DOI: 10.1111/gtc.12385
  • NAID: 120005906898
  • Peer Reviewed / Open Access
• [Journal Article] Virtual nuclear envelope breakdown and its regulators in fission yeast meiosis (2016)
  • Author(s): Asakawa H, Yang H, Hiraoka Y, Haraguchi T.
  • Journal Title: Front. Cell Dev. Volume: 4 Pages: 1-8
  • DOI: 10.3389/fcell.2016.00005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe (2015)
  • Author(s): Haruhiko Asakawa, Chie Mori, Chizuru Ohtsuki, Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: FEBS Open Bio Volume: 5 Issue: 1 Pages: 508-514
  • DOI: 10.1016/j.fob.2015.06.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast. (2015)
  • Author(s): Matsuda A, Chikashige Y, Ding DQ, Ohtsuki C, Mori C, Asakawa H, Kimura H, Haraguchi T, Hiraoka Y.
  • Journal Title: Nature Communication Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms8753
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone H4 acetylation required for chromatin decompaction during DNA replication (2015)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Kimura H, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep12720
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly. (2015)
  • Author(s): Yang H-J, Asakawa H, Haraguchi T, Hiraoka Y.
  • Journal Title: J. Cell Biol. Volume: 211 Issue: 2 Pages: 295-308
  • DOI: 10.1083/jcb.201501035
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena (2015)
  • Author(s): Masaaki Iwamoto, Takako Koujin, Hiroko Osakada, Chie Mori, Tomoko Kojidani, Atsushi Matsuda, Haruhiko Asakawa, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: Journal of Cell Science Volume: 128 Pages: 1812-1823
  • DOI: 10.1242/jcs.167353
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fission yeast meets a legend in Kobe: report of the Eighth International Fission Yeast Meeting. (2015)
  • Author(s): Asakawa H, Yamamoto TG, Hiraoka Y.
  • Journal Title: Genes Cells Volume: 20 Issue: 12 Pages: 967-971
  • DOI: 10.1111/gtc.12307
  • Peer Reviewed / Open Access
• [Journal Article] Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway. (2014)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes Cells Volume: 20 Issue: 3 Pages: 160-172
  • DOI: 10.1111/gtc.12207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 分裂酵母核膜内膜タンパク質Bqt4はLem2と相互作用し、細胞内局在を調整する (2017)
  • Author(s): 衣笠泰葉, 平野泰弘, 淺川東彦, 近重裕次, 原口徳子, 平岡 泰
  • Organizer: 第34回染色体ワークショップ第15回核ダイナミクス研究会
  • Place of Presentation: かずさアカデミアホール（千葉県・木更津市）
  • Year and Date: 2017-01-11
• [Presentation] 分裂酵母に特異的な核膜孔複合体の構造 (2017)
  • Author(s): 淺川東彦、糀谷知子、小坂田裕子、大槻千鶴、長尾恒治、小布施力史、岩本政明、平岡 泰、原口徳子
  • Organizer: 第34回染色体ワークショップ第15回核ダイナミクス研究会
  • Place of Presentation: かずさアカデミアホール（千葉県・木更津市）
  • Year and Date: 2017-01-11
• [Presentation] 生細胞プローブH4K20me1-mintbodyの細胞内標的特異性の検討 (2016)
  • Author(s): 佐藤優子, 淺川東彦, 大槻千鶴, 原口徳子, 平岡 泰, 木村 宏
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 分裂酵母核膜内膜タンパク質Bqt4はLem2と相互作用し、細胞内局在を調整する (2016)
  • Author(s): 衣笠泰葉, 平野泰弘, 淺川東彦, 近重裕次, 原口徳子, 平岡 泰
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Localization of inner nuclear membrane protein Lem2 is regulated by a telomere-anchoring protein Bqt4 in fission yeast. (2016)
  • Author(s): Kinugasa Y, Hirano Y, Asakawa H, Chikashige Y, Haraguchi T, Hiraoka Y.
  • Organizer: 14th International Congress on Yeasts.
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] 分裂酵母に特異的な核膜孔複合体の構造 (2016)
  • Author(s): 淺川東彦、糀谷知子、小坂田裕子、大槻千鶴、長尾恒治、小布施力史、岩本政明、平岡 泰、原口徳子
  • Organizer: 第49回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: シーサイドホテル舞子ビラ（兵庫県・神戸市）
  • Year and Date: 2016-07-07
• [Presentation] 分裂酵母の核膜孔複合体蛋白質のプロテオミクス解析と免疫電子顕微鏡解析 (2016)
  • Author(s): 淺川東彦、糀谷知子、小坂田裕子、大槻千鶴、長尾恒治、小布施力史、岩本政明、平岡 泰、原口徳子
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会（共同開催）
  • Place of Presentation: 松島一の坊（宮城県松島町）
  • Year and Date: 2016-01-13
• [Presentation] Histone H4 acetylation required for chromatin loosening during DNA replication (2015)
  • Author(s): Ruan Kun、山本孝治、淺川東彦、原口徳子、平岡 泰
  • Organizer: 第48回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 広島大学東広島キャンパス（広島県東広島市）
  • Year and Date: 2015-08-31
• [Presentation] Two Nup133-homologs separately function at the cytoplasmic or nuclear side in nuclear pore complex in Schizosaccharomyces pombe. (2015)
  • Author(s): Asakawa H, Kojidani T, Osakada H, Iwamoto M, Nagao K, Obuse C, Hiraoka Y, Haraguchi T.
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: 淡路夢舞台（兵庫県淡路市）
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe. (2015)
  • Author(s): Asakawa H, Mori C, Ohtsuki C, Hiraoka Y, Haraguchi T.
  • Organizer: The eighth International Fission Yeast Meeting (Pombe2015)
  • Place of Presentation: 生田神社会館（神戸市）
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Molecular architecture of the nuclear pore complex in the fission yeast S. pombe (2015)
  • Author(s): Haraguchi T, Asakawa H, Kojidani T, Osakada H, Iwamoto M, Nagao K, Obuse C, Hiraoka Y.
  • Organizer: The eighth International Fission Yeast Meeting (Pombe2015)
  • Place of Presentation: 生田神社会館（神戸市）
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore reassembly. (2015)
  • Author(s): Yang H-J, Asakawa H, Haraguchi T, Hiraoka Y.
  • Organizer: The eighth International Fission Yeast Meeting (Pombe2015)
  • Place of Presentation: 生田神社会館（神戸市）
  • Year and Date: 2015-06-21
  • Int'l Joint Research / Invited
• [Presentation] Codon usage bias affects the mRNA amounts in S. pombe. (2015)
  • Author(s): Kinugasa Y, Asakawa H, Ohtsuki C, Kuroki A, Hiraoka Y.
  • Organizer: The eighth International Fission Yeast Meeting (Pombe2015)
  • Place of Presentation: 生田神社会館（神戸市）
  • Year and Date: 2015-06-21
  • Int'l Joint Research
• [Presentation] 分裂酵母の核膜孔複合体蛋白質Nup131, Nup132の機能解析 (2014)
  • Author(s): 淺川東彦, Hui-ju Yang, 大槻千鶴, 糀谷知子, 森 知栄, 小坂田裕子, 長尾恒治, 小布施力史, 平岡 泰, 原口徳子
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル（広島県廿日市市）
  • Year and Date: 2014-12-16
• [Presentation] Nup132 modulates monopolar spindle attachment through regulating outer kinetochore disassembly at meiotic prophase. (2014)
  • Author(s): Yang HJ, Asakawa H, Haraguchi T, Hiraoka Y.
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル（広島県廿日市市）
  • Year and Date: 2014-12-16
• [Presentation] GFP融合抗体断片の細胞内発現によるタンパク質翻訳後修飾の生細胞観察 (2014)
  • Author(s): 淺川東彦、大槻千鶴、 佐藤優子、木村 宏、平岡 泰
  • Organizer: 第47回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 東京大学弥生講堂（東京都）
  • Year and Date: 2014-09-03
• [Presentation] 分裂酵母における減数分裂期DNA複製の停止による染色体の構造変化 (2014)
  • Author(s): Kun Ruan、山本孝治、浅川東彦、近重裕次、升方久夫、原口徳子、平岡 泰
  • Organizer: 第47回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 東京大学弥生講堂（東京都）
  • Year and Date: 2014-09-02
• [Presentation] Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe. (2014)
  • Author(s): Asakawa H, Yang H-J, Yamamoto TG, Ohtsuki C, Chikashige Y, Sakata-Sogawa K, Tokunaga M, Iwamoto M, Hiraoka Y, Haraguchi T.
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-06-11
• [Presentation] Meiotic progression is impaired in a nuclear envelope tethered-Rpn11 mutant. (2014)
  • Author(s): Yang HJ, Asakawa H, Ohtsuki C, Hiraoka Y.
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-06-11
• [Book] Fission yeast: a laboratory manual. (Hagan I, Carr AM, Grallert A, Nurse P., ed.) "Microscopic Observation of Living Cells Stained with Fluorescent Probes." (2016)
  • Author(s): Asakawa H, Ding D-Q, Haraguchi T, Hiraoka Y.
  • Total Pages: 487
  • Publisher: Cold Spring Harbor Laboratory Press
• [Remarks] 大阪大学大学院生命機能研究科 細胞核ダイナミクス研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/
• [Remarks] 大阪大学大学院生命機能研究科 平岡研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html