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Nobel signaling mechanisms for insulin-dependent glucose uptake in skeletal muscle

Research Project

Project/Area Number 26440103
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionOsaka Prefecture University

Principal Investigator

Satoh Takaya  大阪府立大学, 理学(系)研究科(研究院), 教授 (20251655)

Co-Investigator(Kenkyū-buntansha) 竹中 延之  大阪府立大学, 理学(系)研究科(研究院), 准教授 (20610504)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsインスリン / 骨格筋 / GTP結合蛋白質 / Rac1 / 糖取り込み / 細胞内シグナル伝達 / GTP結合タンパク質 / 糖尿病 / 低分子量GTPアーゼ / グアニンヌクレオチド交換因子
Outline of Final Research Achievements

Insulin regulates the blood glucose level by inducing glucose uptake in skeletal muscle. In this study, we aimed to reveal the role and regulatory mechanisms of the small GTPase Rac1 in insulin-dependent glucose uptake in skeletal muscle by using mouse models. We identified a novel signaling mechanism whereby the guanine nucleotide exchange factor FLJ00068 activates Rac1 downstream of phosphoinositide 3-kinase and the serine/threonine protein kinase Akt2. Furthermore, we found that the small GTPase RalA regulates glucose uptake downstream of Rac1 in skeletal muscle.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Rac1 activation caused by membrane translocation of a guanine nucleotide exchange factor in Akt2-mediated insulin signaling in mouse skeletal muscle.2016

    • Author(s)
      Nobuyuki Takenaka, Yuma Nihata, and Takaya Satoh
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 5 Pages: e0155292-e0155292

    • DOI

      10.1371/journal.pone.0155292

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Immunofluorescent detection of the activation of the small GTPase Rac1 in mouse skeletal muscle fibers.2015

    • Author(s)
      Nobuyuki Takenaka, Yuma Nihata, and Takaya Satoh
    • Journal Title

      Anal. Biochem.

      Volume: 476 Pages: 5-7

    • DOI

      10.1016/j.ab.2014.09.013

    • NAID

      120006895713

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role for RalA downstream of Rac1 in skeletal muscle insulin signalling.2015

    • Author(s)
      Nobuyuki Takenaka, Yukio Sumi, Keiko Matsuda, Junko Fujita, Tetsuya Hosooka, Tetsuya Noguchi, Atsu Aiba, and Takaya Satoh
    • Journal Title

      Biochem. J.

      Volume: 469 Issue: 3 Pages: 445-454

    • DOI

      10.1042/bj20150218

    • NAID

      120006895712

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role of the guanine nucleotide exchange factor in Akt2-mediated plasma membrane translocation of GLUT4 in insulin-stimulated skeletal muscle.2014

    • Author(s)
      Takenaka N, Yasuda N, Nihata Y, Hosooka T, Noguchi T, Aiba A, Satoh T.
    • Journal Title

      Cell Signal.

      Volume: 26 Issue: 11 Pages: 2460-2469

    • DOI

      10.1016/j.cellsig.2014.07.002

    • NAID

      120006895710

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rho GTPases in insulin-stimulated glucose uptake2014

    • Author(s)
      Takaya Satoh
    • Journal Title

      Small GTPases

      Volume: (In press) Issue: 1 Pages: e28102-e28102

    • DOI

      10.4161/sgtp.28102

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular mechanisms for the regulation of insulin-stimulated glucose uptake by small guanosine triphosphatases in skeletal muscle and adipocytes.2014

    • Author(s)
      Takaya Satoh
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 15 Issue: 10 Pages: 18677-18692

    • DOI

      10.3390/ijms151018677

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マウス脂肪組織において内在性低分子量GTP結合蛋白質の活性化を検出する新規手法.2016

    • Author(s)
      植田笙,新畑有麻,竹中延之,佐藤孝哉
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス骨格筋のインスリンシグナル伝達系においてRac1調節因子として機能するグアニンヌクレオチド交換因子の解析.2016

    • Author(s)
      竹中延之,富士田淳子,新畑有麻,佐藤孝哉
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Insulin signal transduction in skeletal muscle.2016

    • Author(s)
      Takaya Satoh, and Nobuyuki Takenaka
    • Organizer
      4th TKU-OPU Joint Symposium
    • Place of Presentation
      Yilan County, Taiwan
    • Year and Date
      2016-11-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Insulin‐like growth factor‐1 induces activation and upregulating of via PI3K/Akt/mTOR pathway in muscular hypertrophy model of C2C12 cells.2015

    • Author(s)
      Akiho Kushiya, Shuji Ueda, Yusuke Kirimura, Yoshiki Katoh, Midori Ishimasa, Takaya Satoh, Minoru Yamanoue, Yasuhito Shirai
    • Organizer
      The 2015 ASCB Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス骨格筋でのインスリン応答性糖取り込みにおけるRac1によるRalAの制御.2015

    • Author(s)
      竹中延之,松田佳子,富士田淳子,佐藤孝哉
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス骨格筋において内在性低分子量GTP結合蛋白質の活性化を検出する新規手法.2015

    • Author(s)
      新畑有麻,竹中延之,佐藤孝哉
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] A role of RhoA activation in IGF-I-induced muscular hypertrophy model.2014

    • Author(s)
      Shuji Ueda, Soichiro Yamada, Yusuke Kirimura, Akiho Kushiya, Minoru Yamanoue, Takaya Satoh, Yasuhito Sirai
    • Organizer
      The 2014 ASCB/IFCB Meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 骨格筋でのインスリン依存性糖取り込みにおけるRac1の活性制御.2014

    • Author(s)
      竹中延之,新畑有麻,北川花穂,佐藤孝哉
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 骨格筋のインスリンシグナル伝達系でRac1調節因子として機能するグアニンヌクレオチド交換因子の解析.2014

    • Author(s)
      新畑有麻,竹中延之,佐藤孝哉
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      公益財団法人国立京都国際会館(京都市)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 骨格筋のインスリン応答性糖取り込みシグナル伝達系においてRac1の下流で機能するRalAの役割.2014

    • Author(s)
      松田佳子,スミ有希雄,竹中延之,佐藤孝哉
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      公益財団法人国立京都国際会館(京都市)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Book] Encyclopedia of Signaling Molecules (2nd Edition)2017

    • Author(s)
      Nobuyuki Takenaka, and Takaya Satoh
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] 大阪公立大学共同出版会2016

    • Author(s)
      佐藤孝哉,竹中延之
    • Total Pages
      197
    • Publisher
      バイオサイエンス実験 入門から応用へ
    • Related Report
      2016 Annual Research Report
  • [Book] 細胞のシグナル伝達 システムとしての共通原理にもとづく理解 (翻訳)2016

    • Author(s)
      佐藤孝哉
    • Total Pages
      415
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2016 Annual Research Report
  • [Book] バイオサイエンス実験 入門から応用へ2016

    • Author(s)
      石原道博、居原秀、恩田真紀、川西優喜、木下誉富、児玉靖司、佐藤孝哉、白石一乗、竹田恵美、竹中延之、円谷健、西野貴子、原正之、藤井郁雄、藤原大佑、森英樹、八木孝司、吉原静江
    • Total Pages
      197
    • Publisher
      大阪公立大学共同出版会
    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪府立大学大学院理学系研究科生物科学専攻分子細胞生物学領域細胞生物学分野ホームページ

    • URL

      http://www.b.s.osakafu-u.ac.jp/~tsato/index.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪府立大学 大学院理学系研究科 生物科学専攻 分子細胞生物学領域 細胞生物学分野

    • URL

      http://www.b.s.osakafu-u.ac.jp/~tsato/index.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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