Regulation of meiosis

• Project/Area Number: 26440104
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Chuo University
• Principal Investigator: Murakami Hiroshi 中央大学, 理工学部, 教授 (80262020)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 減数分裂 / 細胞周期 / チェックポイント / DNA複製 / 遺伝子組換え / 酵母 / 体細胞分裂 / 遺伝子発現 / 転写因子

Outline of Final Research Achievements

We have been screening for the possible candidates of the meiotic DNA replication checkpoint linking pre-meiotic DNA replication and recombination. We found that meiotic forkhead transcription factor Mei4 is required for this checkpoint. However, we have not found any new factors of the meiotic DNA replication checkpoint linking pre-meiotic DNA replication and the first meiotic division.
We have shown that meiRNA which is a non-coding RNA is required for pre-meiotic S phase in the absence of the pheromone signal. We show that meiRNA regulates the expression of the activator of the transcription factor.

Research Products

• [Journal Article] The histone variant H2A.Z promotes initiation of meiotic recombination in fission yeast. (2018)
  • Author(s): Yamada S, Kugou K, Ding DQ, Fujita Y, Hiraoka Y, Murakami H, Ohta K, Yamada T.
  • Journal Title: Nucleic Acids Res. Volume: 46 Issue: 2 Pages: 609-620
  • DOI: 10.1093/nar/gkx1110
  • Peer Reviewed / Open Access
• [Journal Article] Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots. (2017)
  • Author(s): . Yamada, M. Okamura, H. Murakami, K.Ohta, T. Yamada
  • Journal Title: Genetics Volume: in press Issue: 2 Pages: 801-809
  • DOI: 10.1534/genetics.116.197954
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sulfur restriction extends fission yeast chronological lifespan through Ecl1 family genes by downregulation of ribosome (2017)
  • Author(s): Hokuto Ohtsuka*, Masahiro Takinami*, Takafumi Shimasaki, Takahide Hibi, Hiroshi Murakami, and Hirofumi Aiba
  • Journal Title: Molecular Microbiology Volume: 未定 Issue: 1 Pages: 84-97
  • DOI: 10.1111/mmi.13686
  • NAID: 120006349138
  • Peer Reviewed
• [Journal Article] Ecl1 is a zinc binding protein that is involved in the zinc-limitation dependent extension of chronological lifespan in fission yeast. (2017)
  • Author(s): Takafumi Shimasaki, Hokuto Ohtsuka, Chikako Naito, Takeshi Tenno, Hidekazu Hiroaki, Hiroshi Murakami, Hirofumi Aiba
  • Journal Title: Molecular Genetics and Genomics Volume: 292 Issue: 2 Pages: 475-481
  • DOI: 10.1007/s00438-016-1285-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sexual development of Schizosaccharomyces pombe is induced by zinc or iron limitation through Ecl1 family genes. (2015)
  • Author(s): Ohtsuka H, Ishida M, Naito C, Murakami H, Aiba H.
  • Journal Title: Mol Genet Genomics. Volume: 290 Issue: 1 Pages: 173-185
  • DOI: 10.1007/s00438-014-0911-8
  • Peer Reviewed / Open Access
• [Journal Article] A new pma1 mutation identified in a chronologically long-lived fission yeast mutant. (2014)
  • Author(s): Naito C, Ito H, Oshiro T, Ohtsuka H, Murakami H, Aiba H.
  • Journal Title: FEBS Open Bio. Volume: 4 Issue: 1 Pages: 829-833
  • DOI: 10.1016/j.fob.2014.09.006
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of wee1(+) expression during meiosis in fission yeast. (2014)
  • Author(s): Murakami-Tonami Y, Ohtsuka H, Aiba H, Murakami H.
  • Journal Title: Cell Cycle. Volume: 13 Issue: 18 Pages: 2853-2858
  • DOI: 10.4161/15384101.2014.946807
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe. (2014)
  • Author(s): Shimasaki T, Ohtsuka H, Naito C, Murakami H, Aiba H.
  • Journal Title: Mol Genet Genomics. Volume: 289 Issue: 4 Pages: 685-693
  • DOI: 10.1007/s00438-014-0845-1
  • Peer Reviewed / Open Access
• [Journal Article] Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. (2014)
  • Author(s): Murakami-Tonami Y, Kishida S, Takeuchi I, Katou Y, Maris JM, Ichikawa H, Kondo Y, Sekido Y, Shirahige K, Murakami H, Kadomatsu K.
  • Journal Title: Cell Cycle. Volume: 13 Issue: 7 Pages: 1115-1131
  • DOI: 10.4161/cc.27983
  • Peer Reviewed / Open Access
• [Presentation] 減数分裂時のDNA複製と相同組換えを連携するチェックポイント (2017)
  • Author(s): 小菅清二、山田貴富、饗場浩文、村上浩士
  • Organizer: 第５０回酵母遺伝学フォーラム研究報告会
• [Presentation] Regulation of mei4+ expression during mitotic cell cycle in fission yeast (2017)
  • Author(s): Yuuki Akiya, Atsushi Ogihara, Hirofumi Aiba and Hiroshi Murakami
  • Organizer: 9th International Fission yeast meeting,
  • Int'l Joint Research
• [Presentation] 分裂公募S.pombeの減数分裂期組換えに関わるHop1のHORMAドメインの機能解析 (2015)
  • Author(s): 炭谷悠人、山田貴富、太田邦史、村上浩士
  • Organizer: 第３８回日本分子生物学会年会、
  • Place of Presentation: 神戸ポートアイランド、
  • Year and Date: 2015-12-01
• [Book] 化学と生物 (2018)
  • Author(s): 山田貴富、村上浩士
  • Total Pages: 10
  • Publisher: 国際文献社、